The deubiquitinating enzyme AMSH3 is required for intracellular trafficking and vacuole biogenesis in Arabidopsis thaliana.

Détails

ID Serval
serval:BIB_E0FE6690765A
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
The deubiquitinating enzyme AMSH3 is required for intracellular trafficking and vacuole biogenesis in Arabidopsis thaliana.
Périodique
Plant Cell
Auteur⸱e⸱s
Isono E., Katsiarimpa A., Müller I.K., Anzenberger F., Stierhof Y.D., Geldner N., Chory J., Schwechheimer C.
ISSN
1532-298X[electronic], 1040-4651[linking]
Statut éditorial
Publié
Date de publication
2010
Volume
22
Numéro
6
Pages
1826-1837
Langue
anglais
Résumé
Ubiquitination, deubiquitination, and the formation of specific ubiquitin chain topologies have been implicated in various cellular processes. Little is known, however, about the role of ubiquitin in the development of cellular organelles. Here, we identify and characterize the deubiquitinating enzyme AMSH3 from Arabidopsis thaliana. AMSH3 hydrolyzes K48- and K63-linked ubiquitin chains in vitro and accumulates both ubiquitin chain types in vivo. amsh3 mutants fail to form a central lytic vacuole, accumulate autophagosomes, and mis-sort vacuolar protein cargo to the intercellular space. Furthermore, AMSH3 is required for efficient endocytosis of the styryl dye FM4-64 and the auxin efflux facilitator PIN2. We thus present evidence for a role of deubiquitination in intracellular trafficking and vacuole biogenesis.
Mots-clé
Arabidopsis/enzymology, Arabidopsis Proteins/metabolism, Cloning, Molecular, Endocytosis, Mutation, Protein Transport, Ubiquitin/metabolism, Ubiquitin Thiolesterase/metabolism, Ubiquitination, Vacuoles/enzymology
Pubmed
Web of science
Open Access
Oui
Création de la notice
20/01/2011 11:05
Dernière modification de la notice
20/08/2019 16:05
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