In vivo administration of a lentiviral vaccine targets DCs and induces efficient CD8(+) T cell responses.

Détails

ID Serval
serval:BIB_E0506927CBED
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
In vivo administration of a lentiviral vaccine targets DCs and induces efficient CD8(+) T cell responses.
Périodique
The Journal of clinical investigation
Auteur(s)
Esslinger C., Chapatte L., Finke D., Miconnet I., Guillaume P., Lévy F., MacDonald H.R.
ISSN
0021-9738
Statut éditorial
Publié
Date de publication
2003
Peer-reviewed
Oui
Volume
111
Numéro
11
Pages
1673-1681
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
The present study evaluates the potential of third-generation lentivirus vectors with respect to their use as in vivo-administered T cell vaccines. We demonstrate that lentivector injection into the footpad of mice transduces DCs that appear in the draining lymph node and in the spleen. In addition, a lentivector vaccine bearing a T cell antigen induced very strong systemic antigen-specific cytotoxic T lymphocyte (CTL) responses in mice. Comparative vaccination performed in two different antigen models demonstrated that in vivo administration of lentivector was superior to transfer of transduced DCs or peptide/adjuvant vaccination in terms of both amplitude and longevity of the CTL response. Our data suggest that a decisive factor for efficient T cell priming by lentivector might be the targeting of DCs in situ and their subsequent migration to secondary lymphoid organs. The combination of performance, ease of application, and absence of pre-existing immunity in humans make lentivector-based vaccines an attractive candidate for cancer immunotherapy.
Mots-clé
Animals, Antigens, Neoplasm, Bone Marrow/metabolism, CD4-Positive T-Lymphocytes/immunology, CD8-Positive T-Lymphocytes/immunology, CD8-Positive T-Lymphocytes/metabolism, Cell Movement, Cell-Free System, CpG Islands, Dendritic Cells/immunology, Epitopes, Flow Cytometry, Green Fluorescent Proteins, Humans, Immunotherapy, Lentivirus/genetics, Luminescent Proteins/metabolism, Mice, Mice, Transgenic, Microscopy, Fluorescence, Models, Genetic, Neoplasm Proteins/pharmacology, Oligonucleotides/chemistry, Peptides/chemistry, Peptides/metabolism, Polymerase Chain Reaction, Receptors, Antigen, T-Cell/metabolism, T-Lymphocytes, Cytotoxic/immunology, Time Factors, Vaccines/administration &amp, dosage
Pubmed
Web of science
Open Access
Oui
Création de la notice
28/01/2008 12:17
Dernière modification de la notice
20/08/2019 17:04
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