Involvement of endothelial CD44 during in vivo angiogenesis.

Détails

ID Serval
serval:BIB_E00E63F3FABD
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Involvement of endothelial CD44 during in vivo angiogenesis.
Périodique
American Journal of Pathology
Auteur⸱e⸱s
Cao G., Savani R.C., Fehrenbach M., Lyons C., Zhang L., Coukos G., Delisser H.M.
ISSN
0002-9440 (Print)
ISSN-L
0002-9440
Statut éditorial
Publié
Date de publication
2006
Volume
169
Numéro
1
Pages
325-336
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S.Publication Status: ppublish
Résumé
CD44, a cell-surface receptor for hyaluronan, has been implicated in endothelial cell functions, but its role in the formation of blood vessels in vivo has not been established. In CD44-null mice, vascularization of Matrigel implants and tumor and wound angiogenesis were inhibited. Leukocyte accumulation during tumor growth and wound healing in wild-type and CD44-null mice were comparable, and reconstitution of CD44-null mice with wild-type bone marrow did not restore the wild-type phenotype, suggesting that impairments in angiogenesis in CD44-deficient mice are due to the loss of endothelial CD44. Although the cell proliferation, survival, and wound-induced migration of CD44-null endothelial cells were intact, these cells were impaired in their in vitro ability to form tubes. Nascent vessels in Matrigel implants from CD44-null mice demonstrated irregular luminal surfaces characterized by retracted cells and thinned endothelia. Further, an anti-CD44 antibody that disrupted in vitro tube formation induced hemorrhage around Matrigel implants, suggesting that antagonism of endothelial CD44 undermined the integrity of the endothelium of nascent vessels. These data establish a role for CD44 during in vivo angiogenesis and suggest that CD44 may contribute to the organization and/or stability of developing endothelial tubular networks.
Mots-clé
Animals, Antigens, CD44/metabolism, Cell Adhesion, Cell Proliferation, Collagen, Drug Combinations, Endothelial Cells/metabolism, Hyaluronic Acid/metabolism, Immunohistochemistry, Laminin, Mice, Microscopy, Electron, Neoplasms, Experimental/blood supply, Neovascularization, Pathologic, Neovascularization, Physiologic, Proteoglycans, Wound Healing/physiology
Pubmed
Web of science
Création de la notice
14/10/2014 12:43
Dernière modification de la notice
20/08/2019 17:04
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