Enhanced vascular biocompatibility of decellularized xeno-/allogeneic matrices in a rodent model.

Détails

ID Serval
serval:BIB_DF9171332A59
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Enhanced vascular biocompatibility of decellularized xeno-/allogeneic matrices in a rodent model.
Périodique
Cell and tissue banking
Auteur(s)
van Steenberghe M., Schubert T., Guiot Y., Bouzin C., Bollen X., Gianello P.
ISSN
1573-6814 (Electronic)
ISSN-L
1389-9333
Statut éditorial
Publié
Date de publication
06/2017
Peer-reviewed
Oui
Volume
18
Numéro
2
Pages
249-262
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
Glutaraldehyde preservation is the gold standard for cardiovascular biological prosthesis. However, secondary calcifications and the absence of tissue growth remain major limitations. Our study assessed in vitro and in vivo the biocompatibility of human (fascia lata, pericardium) and porcine tissues (pericardium, peritoneum) treated with a physicochemical procedure for decellularization and non-conventional pathogens inactivation. Biopsies were performed before and after treatment to assess decellularization (HE/Dapi staining/DNA quantification/MHC I/alpha gal immunostaining) and mechanical integrity. Forty-five rats received an abdominal aortic patch of native cryopreserved tissues (n = 20), treated tissues (n = 20) or glutaraldehyde-preserved bovine pericardium (GBP, control, n = 5). Grafts were explanted at 4 weeks and processed for HE/von Kossa staining and immunohistochemistries for lymphocytes (CD3)/macrophages (CD68) histomorphometry. 95% of decellularization was obtained for all tissues except for fascia lata (75%). Mechanical properties were slightly altered. In the in vivo model, a significant increase of CD3 and CD68 infiltrations was found in native and control implants in comparison with decellularized tissues (p < 0.05). Calcifications were found in 3 controls. Decellularized tissues were recolonized. GBP showed the most inflammatory response. This physicochemical treatment improves the biocompatibility of selected xeno/allogeneic tissues in comparison with their respective native cryopreserved tissues and with GBP. Incomplete decellularization is associated with a significantly higher inflammatory response. Our treatment is a promising tool in the field of tissue decellularization and tissue banking.

Mots-clé
Animals, Antigens, CD/analysis, Antigens, Differentiation, Myelomonocytic/analysis, Biomechanical Phenomena, Blood Vessel Prosthesis/adverse effects, CD3 Complex/analysis, Cattle, DNA/analysis, Fascia Lata/chemistry, Fascia Lata/cytology, Female, Glutaral/adverse effects, Glutaral/chemistry, Humans, Inflammation/etiology, Male, Materials Testing, Pericardium/chemistry, Pericardium/cytology, Peritoneum/chemistry, Peritoneum/cytology, Rats, Rats, Wistar, Swine, Tissue Scaffolds/chemistry, Allograft, Biocompatibility testing, Cell-free, Glutaraldehyde-stabilized grafts, Rodents, Vascular prosthesis, Xenograft
Pubmed
Web of science
Création de la notice
07/03/2017 20:36
Dernière modification de la notice
20/08/2019 17:03
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