Efficient transduction of dendritic cells and induction of a T-cell response by third-generation lentivectors.

Détails

ID Serval
serval:BIB_DF135A84BCAF
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Efficient transduction of dendritic cells and induction of a T-cell response by third-generation lentivectors.
Périodique
Human gene therapy
Auteur(s)
Esslinger C., Romero P., MacDonald H.R.
ISSN
1043-0342
Statut éditorial
Publié
Date de publication
2002
Peer-reviewed
Oui
Volume
13
Numéro
9
Pages
1091-1100
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
In order to induce a therapeutic T lymphocyte response, recombinant viral vaccines are designed to target professional antigen-presenting cells (APC) such as dendritic cells (DC). A key requirement for their use in humans is safe and efficient gene delivery. The present study assesses third-generation lentivectors with respect to their ability to transduce human and mouse DC and to induce antigen-specific CD8+ T-cell responses. We demonstrate that third-generation lentivectors transduce DC with a superior efficiency compared to adenovectors. The transfer of DC transduced with a recombinant lentivector encoding an antigenic epitope resulted in a strong specific CD8+ T-cell response in mice. The occurrence of lower proportions of nonspecifically activated CD8+ cells suggests a lower antivector immunity of lentivector compared to adenovector. Thus, lentivectors, in addition to their promise for gene therapy of brain disorders might also be suitable for immunotherapy.
Mots-clé
Animals, CD8-Positive T-Lymphocytes/immunology, Cells, Cultured, Dendritic Cells/metabolism, Epitopes, T-Lymphocyte/genetics, Epitopes, T-Lymphocyte/immunology, Genetic Vectors/genetics, Genetic Vectors/metabolism, HIV-1/genetics, Humans, Interferon-gamma/metabolism, Lentivirus/genetics, Lentivirus/metabolism, Mice, Mice, Transgenic, Phenotype, T-Lymphocytes/immunology, Transduction, Genetic
Pubmed
Web of science
Création de la notice
28/01/2008 12:28
Dernière modification de la notice
20/08/2019 17:03
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