Antithrombin is the primary inhibitor of Factor Xa and thrombin. Numerous reports have indicated that age and sex can influence antithrombin levels, but details of the regulation of antithrombin biosynthesis are not known. Thus, a characterization of antithrombin mRNA in eight tissues of young and old male and female rats was carried out. Liver produced the most mRNA, and hence contributes the majority of the plasma antithrombin, followed by the kidneys, with no age or sex related differences in mRNA levels being observed. Elevated amounts of mRNA were detected in aortas of old male rats compared to young ones. No antithrombin mRNA was detected in brain, lung, heart or skeletal muscle, and spleen showed low but variable levels. Plasma antithrombin protein was elevated in old female rats compared to young female or old male rats. These results show that the rat provides a potentially useful system to study the in vivo regulation of antithrombin biosynthesis.