An antisense noncoding RNA enhances translation via localized structural rearrangements of its cognate mRNA.

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Etat: Public
Version: de l'auteur⸱e
Licence: CC BY 4.0
ID Serval
serval:BIB_DE9B64DCF962
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
An antisense noncoding RNA enhances translation via localized structural rearrangements of its cognate mRNA.
Périodique
The Plant cell
Auteur⸱e⸱s
Reis R.S., Deforges J., Schmidt R.R., Schippers JHM, Poirier Y.
ISSN
1532-298X (Electronic)
ISSN-L
1040-4651
Statut éditorial
Publié
Date de publication
31/05/2021
Peer-reviewed
Oui
Volume
33
Numéro
4
Pages
1381-1397
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
A large portion of eukaryotic genes are associated with noncoding, natural antisense transcripts (NATs). Despite sharing extensive sequence complementarity with their sense mRNAs, mRNA-NAT pairs elusively often evade dsRNA-cleavage and siRNA-triggered silencing. More surprisingly, some NATs enhance translation of their sense mRNAs by yet unknown mechanism(s). Here, we show that translation enhancement of the rice (Oryza sativa) PHOSPHATE1.2 (PHO1.2) mRNA is enabled by specific structural rearrangements guided by its noncoding antisense RNA (cis-NATpho1.2). Their interaction in vitro revealed no evidence of widespread intermolecular dsRNA formation, but rather specific local changes in nucleotide base pairing, leading to higher flexibility of PHO1.2 mRNA at a key high guanine-cytosine�(GC) regulatory region inhibiting translation, ∼350-nt downstream of the start codon. Sense-antisense RNA interaction increased formation of the 80S complex in PHO1.2, possibly by inducing structural rearrangement within this inhibitory region, thus making this mRNA more accessible to 60S. This work presents a framework for nucleotide resolution studies of functional mRNA-antisense pairs.
Mots-clé
Gene Expression Regulation, Plant, Oryza/genetics, Plant Proteins/genetics, Plants, Genetically Modified, Protein Biosynthesis, RNA, Antisense/genetics, RNA, Double-Stranded, RNA, Messenger/chemistry, RNA, Messenger/genetics, RNA, Messenger/metabolism, RNA, Untranslated/chemistry, RNA, Untranslated/genetics
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/04/2021 14:49
Dernière modification de la notice
03/03/2022 6:34
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