A novel role for embigin to promote sprouting of motor nerve terminals at the neuromuscular junction.

Détails

ID Serval
serval:BIB_DE63150CD3C6
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
A novel role for embigin to promote sprouting of motor nerve terminals at the neuromuscular junction.
Périodique
Journal of Biological Chemistry
Auteur(s)
Lain E., Carnejac S., Escher P., Wilson M.C., Lømo T., Gajendran N., Brenner H.R.
ISSN
0021-9258 (Print)
ISSN-L
0021-9258
Statut éditorial
Publié
Date de publication
03/2009
Peer-reviewed
Oui
Volume
284
Numéro
13
Pages
8930-8939
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
Publication Status: ppublish
Résumé
Adult skeletal muscle accepts ectopic innervation by foreign motor axons only after section of its own nerve, suggesting that the formation of new neuromuscular junctions is promoted by muscle denervation. With the aim to identify new proteins involved in neuromuscular junction formation we performed an mRNA differential display on innervated versus denervated adult rat muscles. We identified transcripts encoding embigin, a transmembrane protein of the immunoglobulin superfamily (IgSF) class of cell adhesion molecules to be strongly regulated by the state of innervation. In innervated muscle it is preferentially localized to neuromuscular junctions. Forced overexpression in innervated muscle of a full-length embigin transgene, but not of an embigin fragment lacking the intracellular domain, promotes nerve terminal sprouting and the formation of additional acetylcholine receptor clusters at synaptic sites without affecting terminal Schwann cell number or morphology, and it delays the retraction of terminal sprouts following re-innervation of denervated endplates. Conversely, knockdown of embigin by RNA interference in wild-type muscle accelerates terminal sprout retraction, both by itself and synergistically with deletion of neural cell adhesion molecule. These findings indicate that embigin enhances neural cell adhesion molecule-dependent neuromuscular adhesion and thereby modulates neuromuscular junction formation and plasticity.
Mots-clé
Animals, Gene Expression Profiling, Gene Knockdown Techniques, Glycoproteins/genetics, Glycoproteins/metabolism, Mice, Mice, Transgenic, Motor Neurons/cytology, Motor Neurons/metabolism, Muscle, Skeletal/cytology, Muscle, Skeletal/innervation, Neuromuscular Junction/metabolism, Neuronal Plasticity/physiology, Protein Structure, Tertiary/physiology, RNA Interference, Rats, Rats, Wistar, Receptors, Cholinergic/genetics, Receptors, Cholinergic/metabolism, Schwann Cells/cytology, Schwann Cells/metabolism, Transgenes/physiology
Pubmed
Création de la notice
28/02/2015 23:17
Dernière modification de la notice
20/08/2019 17:03
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