Functional analysis of the phospholipase C gene CaPLC1 and two unusual phospholipase C genes, CaPLC2 and CaPLC3, of Candida albicans

Détails

ID Serval
serval:BIB_DE22AE81BCA7
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Functional analysis of the phospholipase C gene CaPLC1 and two unusual phospholipase C genes, CaPLC2 and CaPLC3, of Candida albicans
Périodique
Microbiology
Auteur⸱e⸱s
Kunze  D., Melzer  I., Bennett  D., Sanglard  D., MacCallum  D., Norskau  J., Coleman  D. C., Odds  F. C., Schafer  W., Hube  B.
ISSN
1350-0872 (Print)
Statut éditorial
Publié
Date de publication
10/2005
Volume
151
Numéro
Pt 10
Pages
3381-94
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Oct
Résumé
Phospholipases C are known to be important regulators of cellular processes but may also act as virulence factors of pathogenic microbes. At least three genes in the genome of the human-pathogenic fungus Candida albicans encode phospholipases with conserved phospholipase C (Plc) motifs. None of the deduced protein sequences contain N-terminal signal peptides, suggesting that these phospholipases are not secreted. In contrast to its orthologue in Sacharomyces cerevisiae, CaPLC1 seems to be an essential gene. However, a conditional mutant with reduced transcript levels of CaPLC1 had phenotypes similar to Plc1p-deficient mutants in S. cerevisiae, including reduced growth on media causing increased osmotic stress, on media with a non-glucose carbon source, or at elevated or lower temperatures, suggesting that CaPlc1p, like the Plc1p counterpart in S. cerevisiae, may be involved in multiple cellular processes. Furthermore, phenotypic screening of the heterozygous DeltaCaplc1/CaPLC1 mutant showed additional defects in hyphal formation. The loss of CaPLC1 cannot be compensated by two additional PLC genes of C. albicans (CaPLC2 and CaPLC3) encoding two almost identical phospholipases C with no counterpart in S. cerevisiae but containing structural elements found in bacterial phospholipases C. Although the promoter sequences of CaPLC2 and CaPLC3 differed dramatically, the transcriptional pattern of both genes was similar. In contrast to CaPLC1, CaPLC2 and CaPLC3 are not essential. Although Caplc2/3 mutants had reduced abilities to produce hyphae on solid media, these mutants were as virulent as the wild-type in a model of systemic infection. These data suggest that C. albicans contains two different classes of phospholipases C which are involved in cellular processes but which have no specific functions in pathogenicity.
Mots-clé
Amino Acid Sequence Animals Base Sequence Candida albicans/*enzymology/genetics/*pathogenicity/physiology Candidiasis/microbiology/physiopathology Drosophila Proteins Fungal Proteins/chemistry/genetics/*metabolism Gene Expression Profiling Gene Expression Regulation, Fungal Humans Mice Molecular Sequence Data Mutation Oligonucleotide Array Sequence Analysis Phenotype Phospholipase C/chemistry/*classification/genetics/*metabolism Protein-Serine-Threonine Kinases Sequence Analysis, DNA Transcription, Genetic
Pubmed
Web of science
Création de la notice
25/01/2008 14:40
Dernière modification de la notice
20/08/2019 16:02
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