Toxoplasma gondii Tic20 is essential for apicoplast protein import.

Détails

ID Serval
serval:BIB_DD9FAC813BBB
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Toxoplasma gondii Tic20 is essential for apicoplast protein import.
Périodique
Proceedings of the National Academy of Sciences of the United States of America
Auteur⸱e⸱s
van Dooren G.G., Tomova C., Agrawal S., Humbel B.M., Striepen B.
ISSN
1091-6490 (Electronic)
ISSN-L
0027-8424
Statut éditorial
Publié
Date de publication
2008
Volume
105
Numéro
36
Pages
13574-13579
Langue
anglais
Résumé
Apicomplexan parasites harbor a secondary plastid that has lost the ability to photosynthesize yet is essential for the parasite to multiply and cause disease. Bioinformatic analyses predict that 5-10% of all proteins encoded in the parasite genome function within this organelle. However, the mechanisms and molecules that mediate import of such large numbers of cargo proteins across the four membranes surrounding the plastid remain elusive. In this work, we identify a highly diverged member of the Tic20 protein family in Apicomplexa. We demonstrate that Tic20 of Toxoplasma gondii is an integral protein of the innermost plastid membrane. We engineer a conditional null-mutant and show that TgTic20 is essential for parasite growth. To characterize this mutant functionally, we develop several independent biochemical import assays to reveal that loss of TgTic20 leads to severe impairment of apicoplast protein import followed by organelle loss and parasite death. TgTic20 is the first experimentally validated protein import factor identified in apicoplasts. Our studies provide experimental evidence for a common evolutionary origin of import mechanisms across the innermost membranes of primary and secondary plastids.
Mots-clé
Animals, Cells, Cultured, Humans, Intracellular Membranes/metabolism, Intracellular Membranes/ultrastructure, Intracellular Space/metabolism, Intracellular Space/ultrastructure, Microscopy, Electron, Transmission, Molecular Sequence Data, Protein Transport, Protozoan Proteins/genetics, Protozoan Proteins/metabolism, Toxoplasma/growth & development, Toxoplasma/metabolism
Pubmed
Web of science
Open Access
Oui
Création de la notice
28/02/2012 19:41
Dernière modification de la notice
20/08/2019 17:02
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