Visualizing cellular heterogeneity by quantifying the dynamics of MAPK activity in live mammalian cells with synthetic fluorescent biosensors.

Détails

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Etat: Public
Version: Final published version
Licence: CC BY 4.0
ID Serval
serval:BIB_DD688230BF62
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Visualizing cellular heterogeneity by quantifying the dynamics of MAPK activity in live mammalian cells with synthetic fluorescent biosensors.
Périodique
Heliyon
Auteur⸱e⸱s
Ma M., Bordignon P., Dotto G.P., Pelet S.
ISSN
2405-8440 (Print)
ISSN-L
2405-8440
Statut éditorial
Publié
Date de publication
12/2020
Peer-reviewed
Oui
Volume
6
Numéro
12
Pages
e05574
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: epublish
Résumé
Mitogen-Activated Protein Kinases (MAPKs) control a wide array of cellular functions by transducing extracellular information into defined biological responses. In order to understand how these pathways are regulated, dynamic single cell measurements are highly needed. Fluorescence microscopy is well suited to perform these measurements. However, more dynamic and sensitive biosensors that allow the quantification of signaling activity in living mammalian cells are required. We have engineered a synthetic fluorescent substrate for human MAPKs (ERK, JNK and p38) that relocates from the nucleus to the cytoplasm when phosphorylated by the kinases. We demonstrate that this reporter displays an improved response compared to other relocation biosensors. This assay allows to monitor the heterogeneity in the MAPK response in a population of isogenic cells, revealing pulses of ERK activity upon a physiological EGFR stimulation. We show applicability of this approach to the analysis of multiple cancer cell lines and primary cells as well as its application in vivo to developing tumors. Using this ERK biosensor, dynamic single cell measurements with high temporal resolution can be obtained. These MAPK reporters can be widely applied to the analysis of molecular mechanisms of MAPK signaling in healthy and diseased state, in cell culture assays or in vivo.
Mots-clé
Biochemistry, Cancer research, Cell biology, Fluorescent biosensor, Live-cell imaging, MAPK signaling, Single cells, Systems biology
Pubmed
Web of science
Open Access
Oui
Financement(s)
Université de Lausanne
Création de la notice
17/12/2020 13:11
Dernière modification de la notice
30/04/2021 6:15
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