Stiffness tomography by atomic force microscopy.

Détails

ID Serval
serval:BIB_DD4F0AF48EA4
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Stiffness tomography by atomic force microscopy.
Périodique
Biophysical Journal
Auteur⸱e⸱s
Roduit C., Sekatski S., Dietler G., Catsicas S., Lafont F., Kasas S.
ISSN
0006-3495
Statut éditorial
Publié
Date de publication
2009
Peer-reviewed
Oui
Volume
97
Numéro
2
Pages
674-677
Langue
anglais
Résumé
The atomic force microscope is a convenient tool to probe living samples at the nanometric scale. Among its numerous capabilities, the instrument can be operated as a nano-indenter to gather information about the mechanical properties of the sample. In this operating mode, the deformation of the cantilever is displayed as a function of the indentation depth of the tip into the sample. Fitting this curve with different theoretical models permits us to estimate the Young's modulus of the sample at the indentation spot. We describe what to our knowledge is a new technique to process these curves to distinguish structures of different stiffness buried into the bulk of the sample. The working principle of this new imaging technique has been verified by finite element models and successfully applied to living cells.
Mots-clé
animals , biomechanics , cell line , cell survival, cytoskeleton/chemistry , cytoskeleton/metabolisme , elastic modulus , elasticity , humans , microscopy , atomic force , nanotechnology , rats , reproducibility of results
Pubmed
Web of science
Open Access
Oui
Création de la notice
15/09/2009 8:44
Dernière modification de la notice
20/08/2019 17:02
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