Simultaneous HPLC analysis of the hypnotic benzodiazepines nitrazepam, estazolam, flunitrazepam, and triazolam in plasma.

Détails

ID Serval
serval:BIB_DCEC17E65323
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Simultaneous HPLC analysis of the hypnotic benzodiazepines nitrazepam, estazolam, flunitrazepam, and triazolam in plasma.
Périodique
Journal of Analytical Toxicology
Auteur(s)
Boukhabza A., Lugnier A.A., Kintz P., Mangin P.
ISSN
0146-4760[print], 0146-4760[linking]
Statut éditorial
Publié
Date de publication
1991
Volume
15
Numéro
6
Pages
319-322
Langue
anglais
Résumé
A new analytical methodology was developed for simultaneous high-performance liquid chromatographic quantitation of nitrazepam, estazolam, flunitrazepam, and triazolam in plasma. After addition of the internal standard nor-prazepam, biological samples and calibration standards were extracted at basic pH into diethyl ether-methylene chloride (2:1, v/v). The reconstituted extract was separated on a reversed-phase Nova Pak C18 column with acidic buffer (6mM)-acetonitrile-methanol (64:23:13, v/v) as mobile phase. The detection was performed at 242 nm. Within-run and day-to-day precision values were about 2-8%. The method is thus sensitive and reproducible for use in clinical and experimental toxicokinetic studies.
Mots-clé
Chromatography, High Pressure Liquid, Estazolam/blood, Flunitrazepam/blood, Humans, Nitrazepam/blood, Triazolam/blood
Pubmed
Création de la notice
15/10/2010 15:18
Dernière modification de la notice
20/08/2019 16:01
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