Plasminogen activators play an essential role in extracellular-matrix invasion by lymphoblastic T cells

Détails

ID Serval
serval:BIB_DCEA1CAB4A94
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Plasminogen activators play an essential role in extracellular-matrix invasion by lymphoblastic T cells
Périodique
International Journal of Cancer
Auteur(s)
Reiter  L. S., Spertini  O., Kruithof  E. K.
ISSN
0020-7136 (Print)
Statut éditorial
Publié
Date de publication
02/1997
Volume
70
Numéro
4
Pages
461-466
Langue
anglais
Notes
Journal Article Research Support, Non-U.S. Gov't --- Old month value: Feb 7
Résumé
Involvement of extravascular sites, in particular infiltration of the central nervous system, is a frequent complication of T-lymphoblastic leukemia and contributes to leukemia-associated morbidity. In this report, we studied the contribution of plasminogen activators to the invasive properties of 7 human T-cell lines in a model of transmigration through an extracellular matrix. The T-cell lines were found to express either urokinase (u-PA) and high levels of u-PA receptor or tissue-type plasminogen activator (t-PA) and low levels of u-PA receptor. The rate of transmigration was consistently higher for u-PA-expressing cells than for t-PA-expressing cells. PA-inhibitor type 1 (PAI-1) was detected in the conditioned medium of one cell line and PAI-2 was detected in cell extracts from 6 lines. The transmigration of 6 out of 7 cell lines was inhibited by trasylol, an inhibitor of plasmin, by an excess of exogenous PAI-1 or PAI-2, and by antibodies to the particular PA type expressed by the cells. Partial inhibition of transmigration by the amino-terminal fragment of u-PA implies that the u-PA receptor contributes to transmigration. Thus, the transmigration of T-leukemia cells through a barrier of extracellular matrix requires PA-dependent proteolysis, which can be provided either by u-PA or t-PA. Specific inhibition of the PA system could provide a means to inhibit tissue invasion by T lymphoblastic cells.
Mots-clé
Antigens, CD/metabolism Humans Leukemia, T-Cell/enzymology/immunology/*pathology *Neoplasm Invasiveness Neoplasm Proteins/*physiology Plasminogen Activators/*physiology Tissue Plasminogen Activator/physiology Tumor Cells, Cultured Urinary Plasminogen Activator/physiology
Pubmed
Web of science
Open Access
Oui
Création de la notice
25/01/2008 15:31
Dernière modification de la notice
20/08/2019 16:01
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