Leishmania major: histone H1 gene expression from the sw3 locus.

Détails

ID Serval
serval:BIB_DCC78D24A5DA
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Leishmania major: histone H1 gene expression from the sw3 locus.
Périodique
Experimental Parasitology
Auteur(s)
Belli S., Formenton A., Noll T., Ivens A., Jacquet R., Desponds C., Hofer D., Fasel N.
ISSN
0014-4894 (Print)
ISSN-L
0014-4894
Statut éditorial
Publié
Date de publication
1999
Volume
91
Numéro
2
Pages
151-160
Langue
anglais
Résumé
Histone H1 in the parasitic protozoan Leishmania is a developmentally regulated protein encoded by the sw3 gene. Here we report that histone H1 variants exist in different Leishmania species and strains of L. major and that they are encoded by polymorphic genes. Amplification of the sw3 gene from the genome of three strains of L. major gave rise to different products in each strain, suggesting the presence of a multicopy gene family. In L. major, these genes were all restricted to a 50-kb Bg/II fragment found on a chromosomal band of 1.3 Mb (chromosome 27). The detection of RFLPs in this locus demonstrated its heterogeneity within several species and strains of Leishmania. Two different copies of sw3 (sw3.0 and sw3.1) were identified after screening a cosmid library containing L. major strain Friedlin genomic DNA. They were identical in their 5' UTRs and open reading frames, but differed in their 3' UTRs. With respect to the originally cloned copy of sw3 from L. major strain LV39, their open reading frames lacked a repeat unit of 9 amino acids. Immunoblots of L. guyanensis parasites transfected with these cosmids revealed that both copies could give rise to the histone H1 protein. The characterization of this locus will now make possible a detailed analysis of the function of histone H1 in Leishmania, as well as permit the dissection of the molecular mechanisms governing the developmental regulation of the sw3 gene.
Mots-clé
Amino Acid Sequence, Animals, Base Sequence, Blotting, Southern, Blotting, Western, Cloning, Molecular, DNA Primers, DNA, Protozoan/chemistry, Electrophoresis, Gel, Pulsed-Field, Electrophoresis, Polyacrylamide Gel, Gene Expression Regulation, Developmental, Gene Library, Histones/chemistry, Histones/genetics, Leishmania major/chemistry, Leishmania major/genetics, Molecular Sequence Data, Polymerase Chain Reaction, Polymorphism, Restriction Fragment Length, Rabbits, Restriction Mapping, Sequence Analysis, DNA, Sequence Homology, Amino Acid
Pubmed
Web of science
Création de la notice
24/01/2008 15:02
Dernière modification de la notice
20/08/2019 16:01
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