Dispersed mammary gland epithelial cells. I. Isolation and separation procedures
Détails
ID Serval
serval:BIB_DC486E0D8DE8
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Dispersed mammary gland epithelial cells. I. Isolation and separation procedures
Périodique
Journal of Cell Biology
ISSN
0021-9525 (Print)
Statut éditorial
Publié
Date de publication
02/1977
Volume
72
Numéro
2
Pages
390-405
Notes
Journal Article
Research Support, U.S. Gov't, P.H.S. --- Old month value: Feb
Research Support, U.S. Gov't, P.H.S. --- Old month value: Feb
Résumé
The mammary gland from midpregnant rabbits has been dissociated into individual cells by enzymatic digestion, divalent cation chelation, and gentle shearing. A heterogeneous cell population is obtained, comprising approximately 60% parenchymal cells, approximately 10% myoepithelial cells, and approximately 30% connective tissue cells, including fibroblasts, plasma cells, and microphages. The epithelial cells are characterized by the presence of fat droplets, which in 65% of the cells form large supranuclear vacuoles. Their buoyant density is less than 1.045, allowing their separation from myoepithelial cells and connective tissue cells by isopycnic centrifugation in a density gradient. The homogeneity of the epithelial cell fraction has been assessed by light and electron microscopy. The cells are viable and functionally active as indicated by their ability to exclude vital dyes, incorporate labeled precursors, consume oxygen, maintain intracellular Na+ and K+ concentrations, and retain their structural integrity. In addition, when cultured in Petri dishes, the cells grow as a monolayer, reestablish junctional complexes and retain cell polarity.
Mots-clé
Animals
Cell Nucleus/ultrastructure
Cell Separation/*methods
Centrifugation, Isopycnic
Endoplasmic Reticulum/ultrastructure
Epithelial Cells
Epithelium/metabolism
Female
Golgi Apparatus/ultrastructure
Intercellular Junctions/ultrastructure
Leucine/metabolism
Mammary Glands, Animal/*cytology/metabolism
Oxygen Consumption
Potassium/analysis
Pregnancy
Rabbits
Sodium/analysis
Thymidine/metabolism
Pubmed
Web of science
Open Access
Oui
Création de la notice
25/01/2008 15:05
Dernière modification de la notice
20/08/2019 16:01