Haemodialysis acutely reduces the plasma levels of ADMA without reversing impaired NO-dependent vasodilation.

Détails

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Etat: Public
Version: Final published version
ID Serval
serval:BIB_DB929680ECA0
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Haemodialysis acutely reduces the plasma levels of ADMA without reversing impaired NO-dependent vasodilation.
Périodique
Clinical Science
Auteur(s)
Engelberger R.P., Teta D., Henry H., De Senarclens O., Dischl B., Liaudet L., Burnier M., Waeber B., Feihl F.
ISSN
1470-8736[electronic]
Statut éditorial
Publié
Date de publication
2009
Volume
117
Numéro
7-8
Pages
293-303
Langue
anglais
Résumé
End-stage renal disease patients have endothelial dysfunction and high plasma levels of ADMA (asymmetric omega-NG,NG-dimethylarginine), an endogenous inhibitor of NOS (NO synthase). The actual link between these abnormalities is controversial. Therefore, in the present study, we investigated whether HD (haemodialysis) has an acute impact on NO-dependent vasodilation and plasma ADMA in these patients. A total of 24 patients undergoing maintenance HD (HD group) and 24 age- and gender-matched healthy controls (Control group) were enrolled. The increase in forearm SkBF (skin blood flow) caused by local heating to 41 degrees C (SkBF41), known to depend on endothelial NO production, was determined with laser Doppler imaging. SkBF41 was expressed as a percentage of the vasodilatory reserve obtained from the maximal SkBF induced by local heating to 43 degrees C (independent of NO). In HD patients, SkBF41 was assessed on two successive HD sessions, once immediately before and once immediately after HD. Plasma ADMA was assayed simultaneously with MS/MS (tandem MS). In the Control group, SkBF41 was determined twice, on two different days, and plasma ADMA was assayed once. In HD patients, SkBF41 was identical before (82.2+/-13.1%) and after (82.7+/-12.4%) HD, but was lower than in controls (day 1, 89.6+/-6.1; day 2, 89.2+/-6.9%; P<0.01 compared with the HD group). In contrast, plasma ADMA was higher before (0.98+/-0.17 micromol/l) than after (0.58+/-0.10 micromol/l; P<0.01) HD. ADMA levels after HD did not differ from those obtained in controls (0.56+/-0.11 micromol/l). These findings show that HD patients have impaired NO-dependent vasodilation in forearm skin, an abnormality not acutely reversed by HD and not explained by ADMA accumulation.
Mots-clé
Asymmetric Omega-N-G,N-G-Dimethylarginine (Adma), Chronic Haemodialysis, Endothelium-Dependent Vasodilation, Endstage Renal Disease, Nitric Oxide, Skin Blood Flow, Chronic Kidney-Disease, Asymmetric Dimethylarginine Adma, Chronic-Renal-Failure, Skin Blood-Flow, Nitric-Oxide Synthase, Endothelial Function, L-Arginine, In-Vivo, Cutaneous Vasodilation, Cardiovascular-Disease
Pubmed
Web of science
Open Access
Oui
Création de la notice
29/10/2009 10:47
Dernière modification de la notice
20/08/2019 17:00
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