Gene expression in human hepatocytes in suspension after isolation is similar to the liver of origin, is not affected by hepatocyte cold storage and cryopreservation, but is strongly changed after hepatocyte plating.

Détails

ID Serval
serval:BIB_DB8DBF7B2930
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Gene expression in human hepatocytes in suspension after isolation is similar to the liver of origin, is not affected by hepatocyte cold storage and cryopreservation, but is strongly changed after hepatocyte plating.
Périodique
Drug Metabolism and Disposition
Auteur(s)
Richert L., Liguori M.J., Abadie C., Heyd B., Mantion G., Halkic N., Waring J.F.
ISSN
0090-9556
Statut éditorial
Publié
Date de publication
2006
Peer-reviewed
Oui
Volume
34
Numéro
5
Pages
870-879
Langue
anglais
Notes
Publication types: In Vitro ; Journal Article
Résumé
Isolated primary human hepatocytes are a well accepted system for evaluating pharmacological and toxicological effects in humans. However, questions remain regarding how culturing affects the liver-specific functions of the hepatocytes. In addition, cryopreservation could also potentially affect the differentiation state of the hepatocytes. The first aim of the present study was to compare gene expression in freshly isolated primary hepatocytes to that of the liver of origin and to evaluate the expression changes occurring after cryopreservation/thawing, both when maintained in suspension and after plating. The second aim of the present study was to evaluate gene expression in hepatocytes after cold storage of suspensions up to 24 h compared with freshly isolated hepatocytes in suspension. Our results show that the gene expression in freshly isolated human hepatocytes in suspension after isolation is similar to that of the liver of origin. Furthermore, gene expression in primary human hepatocytes in suspension is not affected by hepatocyte cold storage and cryopreservation. However, the gene expression is profoundly affected in monolayer cultures after plating. Specifically, gene expression changes were observed in cultured relative to suspensions of human hepatocytes that are involved in cellular processes such as phase I/II metabolism, basolateral and canalicular transport systems, fatty acid and lipid metabolism, apoptosis, and proteasomal protein recycling. An oxidative stress response may be partially involved in these changes in gene expression. Taken together, these results may aid in the interpretation of data collected from human hepatocyte experiments and suggest additional utility for cold storage and cryopreservation of hepatocytes.
Mots-clé
Cell Separation, Cells, Cultured, Cryopreservation, Cytological Techniques/methods, Gene Expression/physiology, Hepatocytes/metabolism, Humans, Liver/metabolism, Oligonucleotide Array Sequence Analysis, RNA/biosynthesis, RNA/isolation &amp, purification, Tissue Preservation
Pubmed
Web of science
Création de la notice
18/02/2008 13:30
Dernière modification de la notice
20/08/2019 16:00
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