Enhanced immunogenicity of microencapsulated tetanus toxoid with stabilizing agents

Détails

ID Serval
serval:BIB_DAE8D7E9E478
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Enhanced immunogenicity of microencapsulated tetanus toxoid with stabilizing agents
Périodique
Pharmaceutical Research
Auteur(s)
Audran  R., Men  Y., Johansen  P., Gander  B., Corradin  G.
ISSN
0724-8741 (Print)
Statut éditorial
Publié
Date de publication
07/1998
Volume
15
Numéro
7
Pages
1111-6
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jul
Résumé
PURPOSE: Antigenic proteins encapsulated in biodegradable polyester microspheres (MS) can slowly denature or aggregate, which results in decreased antigenicity. In this study, we have evaluated the ability of co-encapsulated additives to protect against the loss of tetanus toxoid (TT) antigenicity. METHODS: Antibody responses were analyzed after immunization of mice with TT microencapsulated in the presence of additives (TT-MS-additive). RESULTS: Immunization with TT-MS-additives gave rise to higher responses than those obtained in the absence of additive. BSA, trehalose. Gamma-hydroxypropylcyclodextrin and calcium salts preserved the immunogenicity of the incorporated antigen with the highest efficacy. Sustained responses were obtained with mixtures of fast and slowly releasing TT-MS containing BSA plus trehalose or calcium salts. CONCLUSIONS: The selected additives may stabilize the antigen in MS during storage and rehydration in body fluids. Regulated antigen release from MS-based vaccines permits a reduction of the antigen dose and optimization of single-dose vaccine formulations.
Mots-clé
Animals Antibodies, Bacterial/biosynthesis/blood Capsules Chemistry, Pharmaceutical Enzyme-Linked Immunosorbent Assay Excipients/*administration & dosage/chemistry Female Lactic Acid/chemistry Mice Mice, Inbred BALB C Microspheres Polyglycolic Acid/chemistry Polymers/chemistry Tetanus Toxoid/*administration & dosage/chemistry/*immunology
Pubmed
Web of science
Création de la notice
24/01/2008 15:55
Dernière modification de la notice
20/08/2019 17:00
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