Differential changes in synaptic proteins in the Alzheimer frontal cortex with marked increase in PSD-95 postsynaptic protein

Détails

ID Serval
serval:BIB_DACEB4A886F8
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Differential changes in synaptic proteins in the Alzheimer frontal cortex with marked increase in PSD-95 postsynaptic protein
Périodique
Journal of Alzheimer's Disease
Auteur(s)
Leuba G., Savioz A., Vernay A., Carnal B., Kraftsik R., Tardif E., Riederer I., Riederer B. M.
ISSN
1387-2877
Statut éditorial
Publié
Date de publication
2008
Peer-reviewed
Oui
Volume
15
Numéro
1
Pages
139-151
Langue
anglais
Notes
SAPHIRID:69075
Résumé
We investigated how synaptic plasticity is related to the neurodegeneration process in the human dorsolateral prefrontal cortex. Pre- and postsynaptic proteins of Brodmann's area 9 from patients with Alzheimer's disease (AD) and age-matched controls were quantified by immunohistochemical methods and Western blots. The main finding was a significant increase in the expression of postsynaptic density protein PSD-95 in AD brains, revealed on both sections and immunoblots, while the expression of spinophilin, associated to spines, remained quantitatively unchanged despite qualitative changes with age and disease. Presynaptic protein alpha-synuclein indicated an increased immunohistochemical level, while synaptophysin remained unchanged. MAP2, a somatodendritic microtubule protein, as well as AD markers such as amyloid-beta protein and phosphorylated protein tau showed an increased expression on immunosections in AD. Altogether these changes suggest neuritic and synaptic reorganization in the process of AD. In particular, the significant increase in PSD-95 expression suggests a change in NMDA receptors trafficking and may represent a novel marker of functional significance for the disease.
Mots-clé
Adult Aged Aged, 80 and over Alzheimer Disease/*metabolism Blotting, Western Electrophoresis Female Frontal Lobe/*metabolism Humans Immunohistochemistry Intracellular Signaling Peptides and Proteins/*metabolism Male Membrane Proteins/*metabolism Middle Aged Synapses/*metabolism
Pubmed
Web of science
Création de la notice
30/01/2009 11:13
Dernière modification de la notice
20/08/2019 16:59
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