Distribution and processing of the polymeric immunoglobulin receptor in the rat hepatocyte: morphological and biochemical characterization of subcellular fractions

Détails

ID Serval
serval:BIB_DAB0D8897F92
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Distribution and processing of the polymeric immunoglobulin receptor in the rat hepatocyte: morphological and biochemical characterization of subcellular fractions
Périodique
Journal of Histochemistry and Cytochemistry
Auteur⸱e⸱s
Solari  R., Racine  L., Tallichet  C., Kraehenbuhl  J. P.
ISSN
0022-1554 (Print)
Statut éditorial
Publié
Date de publication
01/1986
Volume
34
Numéro
1
Pages
17-23
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jan
Résumé
The transepithelial transport of polymeric immunoglobulins is an essential process in the mucosal immune system. Transport across the epithelial cells of mucous or exocrine glands is affected by an integral membrane glycoprotein receptor known as membrane secretory component (SCm) or as polymeric immunoglobulin receptor (pIgR). This receptor binds polymeric immunoglobulins at the basolateral cell surface and mediates their transcellular translocation and their release from the apical plasma membrane into external secretions. Release depends on cleavage of the membrane-anchoring domain of the receptor, resulting in liberation of polymeric immunoglobulin bound to the ectoplasmic domain of the receptor (secreted SC or SCs) into extracellular secretions. Using a monoclonal antibody directed against the cytoplasmic tail of the receptor and a polyclonal antibody directed against the secreted ectoplasmic domain, we have combined cell fractionation and Western blotting techniques to examine the fate of these receptor domains in the hepatocyte. In this study, we characterize biochemically and morphologically the various subcellular components separated by our fractionation scheme, and correlate this with biochemical analysis of the receptor in each fraction.
Mots-clé
Animals Cell Fractionation Golgi Apparatus/analysis *Immunoglobulin A Immunoglobulin Fragments/*analysis Liver/*analysis/ultrastructure Microscopy, Electron Rats Receptors, Immunologic/*analysis Secretory Component/*analysis
Pubmed
Web of science
Création de la notice
25/01/2008 15:06
Dernière modification de la notice
20/08/2019 15:59
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