The IBO germination quantitative trait locus encodes a phosphatase 2C-related variant with a nonsynonymous amino acid change that interferes with abscisic acid signaling.

Détails

ID Serval
serval:BIB_DA9042C6DC89
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
The IBO germination quantitative trait locus encodes a phosphatase 2C-related variant with a nonsynonymous amino acid change that interferes with abscisic acid signaling.
Périodique
New Phytologist
Auteur⸱e⸱s
Amiguet-Vercher A., Santuari L., Gonzalez-Guzman M., Depuydt S., Rodriguez P.L., Hardtke C.S.
ISSN
1469-8137 (Electronic)
ISSN-L
0028-646X
Statut éditorial
Publié
Date de publication
2015
Volume
205
Numéro
3
Pages
1076-1082
Langue
anglais
Résumé
Natural genetic variation is crucial for adaptability of plants to different environments. Seed dormancy prevents precocious germination in unsuitable conditions and is an adaptation to a major macro-environmental parameter, the seasonal variation in temperature and day length. Here we report the isolation of IBO, a quantitative trait locus (QTL) that governs c. 30% of germination rate variance in an Arabidopsis recombinant inbred line (RIL) population derived from the parental accessions Eilenburg-0 (Eil-0) and Loch Ness-0 (Lc-0). IBO encodes an uncharacterized phosphatase 2C-related protein, but neither the Eil-0 nor the Lc-0 variant, which differ in a single amino acid, have any appreciable phosphatase activity in in vitro assays. However, we found that the amino acid change in the Lc-0 variant of the IBO protein confers reduced germination rate. Moreover, unlike the Eil-0 variant of the protein, the Lc-0 variant can interfere with the activity of the phosphatase 2C ABSCISIC ACID INSENSITIVE 1 in vitro. This suggests that the Lc-0 variant possibly interferes with abscisic acid signaling, a notion that is supported by physiological assays. Thus, we isolated an example of a QTL allele with a nonsynonymous amino acid change that might mediate local adaptation of seed germination timing.
Mots-clé
abscisic acid (ABA), Arabidopsis thaliana, IBO, natural variation, phosphatase 2C (PP2C), quantitative trait locus (QTL), REDUCED DORMANCY 5 (RDO5)
Pubmed
Web of science
Création de la notice
13/02/2015 16:56
Dernière modification de la notice
20/08/2019 15:59
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