Caveolin expression changes in the neurovascular unit after juvenile traumatic brain injury: signs of blood-brain barrier healing?

Détails

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Etat: Public
Version: de l'auteur
ID Serval
serval:BIB_D9E7A2FD13CD
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Caveolin expression changes in the neurovascular unit after juvenile traumatic brain injury: signs of blood-brain barrier healing?
Périodique
Neuroscience
Auteur(s)
Badaut J., Ajao D.O., Sorensen D.W., Fukuda A.M., Pellerin L.
ISSN
1873-7544 (Electronic)
ISSN-L
0306-4522
Statut éditorial
Publié
Date de publication
29/01/2015
Peer-reviewed
Oui
Volume
285
Pages
215-226
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
Publication Status: ppublish
Résumé
Traumatic brain injury (TBI) is one of the major causes of death and disability in pediatrics, and results in a complex cascade of events including the disruption of the blood-brain barrier (BBB). A controlled-cortical impact on post-natal 17-day-old rats induced BBB disruption by IgG extravasation from 1 to 3 days after injury and returned to normal at day 7. In parallel, we characterized the expression of three caveolin isoforms, caveolin 1 (cav-1), caveolin 2 (cav-2) and caveolin 3 (cav-3). While cav-1 and cav-2 are expressed on endothelial cells, both cav-1 and cav-3 were found to be present on reactive astrocytes, in vivo and in vitro. Following TBI, cav-1 expression was increased in blood vessels at 1 and 7 days in the perilesional cortex. An increase of vascular cav-2 expression was observed 7 days after TBI. In contrast, astrocytic cav-3 expression decreased 3 and 7 days after TBI. Activation of endothelial nitric oxide synthase (eNOS) (via its phosphorylation) was detected 1 day after TBI and phospho-eNOS was detected both in association with blood vessels and with astrocytes. The molecular changes involving caveolins occurring in endothelial cells following juvenile-TBI might participate, independently of eNOS activation, to a mechanism of BBB repair while, they might subserve other undefined roles in astrocytes.

Mots-clé
Animals, Astrocytes/metabolism, Astrocytes/pathology, Blood-Brain Barrier/metabolism, Blood-Brain Barrier/pathology, Brain/blood supply, Brain/growth & development, Brain/metabolism, Brain/pathology, Brain Injuries/metabolism, Brain Injuries/pathology, Caveolin 1/metabolism, Caveolin 2/metabolism, Caveolin 3/metabolism, Cells, Cultured, Disease Models, Animal, Endothelium, Vascular/metabolism, Endothelium, Vascular/pathology, Male, Nitric Oxide Synthase Type III/metabolism, Phosphorylation, Rats, Sprague-Dawley, Time Factors
Pubmed
Open Access
Oui
Création de la notice
15/12/2014 16:42
Dernière modification de la notice
20/08/2019 15:59
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