Viral Q beta RNA as a high expression vector for mRNA translation in a cell-free system.

Détails

ID Serval
serval:BIB_D9CBF28FFC6D
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Viral Q beta RNA as a high expression vector for mRNA translation in a cell-free system.
Périodique
FEBS Letters
Auteur⸱e⸱s
Katanaev V.L., Kurnasov O.V., Spirin A.S.
ISSN
0014-5793 (Print)
ISSN-L
0014-5793
Statut éditorial
Publié
Date de publication
1995
Volume
359
Numéro
1
Pages
89-92
Langue
anglais
Résumé
Dihydrofolate reductase (DHFR) mRNA was inserted into Q beta phage RNA instead of its coat protein cistron. Translation of this recombinant mRNA in the Escherichia coli cell-free system resulted in the synthesis of DHFR, which was two orders of magnitude higher than that in the case of translation of the control DHFR mRNA. Additionally, it resulted in a significantly enhanced synthesis of Q beta replicase as compared with its synthesis when the original Q beta RNA was used.
Mots-clé
Allolevivirus/genetics, Capsid/genetics, Escherichia coli/genetics, Gene Transfer Techniques, Genes, Genetic Vectors, Protein Biosynthesis, RNA, Messenger/genetics, RNA, Viral/genetics, Recombinant Proteins/biosynthesis, Tetrahydrofolate Dehydrogenase/biosynthesis, Tetrahydrofolate Dehydrogenase/genetics
Pubmed
Web of science
Open Access
Oui
Création de la notice
09/07/2012 9:16
Dernière modification de la notice
20/08/2019 15:59
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