An allele-specific, stochastic gene expression process controls the expression of multiple Ly49 family genes and generates a diverse, MHC-specific NK cell receptor repertoire.

Détails

ID Serval
serval:BIB_D956B88AD93C
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
An allele-specific, stochastic gene expression process controls the expression of multiple Ly49 family genes and generates a diverse, MHC-specific NK cell receptor repertoire.
Périodique
European journal of immunology
Auteur(s)
Held W., Kunz B.
ISSN
0014-2980
Statut éditorial
Publié
Date de publication
1998
Peer-reviewed
Oui
Volume
28
Numéro
8
Pages
2407-2416
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Mouse NK cells express MHC class I-specific inhibitory Ly49 receptors. Since these receptors display distinct ligand specificities and are clonally distributed, their expression generates a diverse NK cell receptor repertoire specific for MHC class I molecules. We have previously found that the Dd (or Dk)-specific Ly49A receptor is usually expressed from a single allele. However, a small fraction of short-term NK cell clones expressed both Ly49A alleles, suggesting that the two Ly49A alleles are independently and randomly expressed. Here we show that the genes for two additional Ly49 receptors (Ly49C and Ly49G2) are also expressed in a (predominantly) mono-allelic fashion. Since single NK cells can co-express multiple Ly49 receptors, we also investigated whether mono-allelic expression from within the tightly linked Ly49 gene cluster is coordinate or independent. Our clonal analysis suggests that the expression of alleles of distinct Ly49 genes is not coordinate. Thus Ly49 alleles are apparently independently and randomly chosen for stable expression, a process that directly restricts the number of Ly49 receptors expressed per single NK cell. We propose that the Ly49 receptor repertoire specific for MHC class I is generated by an allele-specific, stochastic gene expression process that acts on the entire Ly49 gene cluster.
Mots-clé
Alleles, Animals, Antigens, Ly, Base Sequence, Clone Cells, DNA Primers/genetics, Gene Expression, Histocompatibility Antigens Class I, Killer Cells, Natural/immunology, Lectins, C-Type, Membrane Glycoproteins/genetics, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Multigene Family, NK Cell Lectin-Like Receptor Subfamily A, Polymerase Chain Reaction, Receptors, Immunologic/genetics, Receptors, NK Cell Lectin-Like, Stochastic Processes
Pubmed
Web of science
Open Access
Oui
Création de la notice
17/01/2008 15:24
Dernière modification de la notice
20/08/2019 15:58
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