Plasmodium vivax thrombospondin related adhesion protein: immunogenicity and protective efficacy in rodents and Aotus monkeys

Détails

ID Serval
serval:BIB_D92A17622536
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Plasmodium vivax thrombospondin related adhesion protein: immunogenicity and protective efficacy in rodents and Aotus monkeys
Périodique
Memorias do Instituto Oswaldo Cruz
Auteur(s)
Castellanos  A., Arevalo-Herrera  M., Restrepo  N., Gulloso  L., Corradin  G., Herrera  S.
ISSN
0074-0276 (Print)
Statut éditorial
Publié
Date de publication
06/2007
Volume
102
Numéro
3
Pages
411-6
Notes
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't --- Old month value: Jun
Résumé
The thrombospondin related adhesion protein (TRAP) is a malaria pre-erythrocytic antigen currently pursued as malaria vaccine candidate to Plasmodium falciparum. In this study, a long synthetic peptide (LSP) representing a P. vivax TRAP fragment involved in hepatocyte invasion was formulated in both Freund and Montanide ISA 720 adjutants and administered by IM and subcutaneous routes to BALB/c mice and Aotus monkeys. We measured specific humoral immune responses in both animal species and performed a sporozoite challenge in Aotus monkeys to assess the protective efficacy of the vaccine. After immunization both mice and Aotus seroconverted as shown by ELISA, and the specific anti-peptide antibodies cross reacted with the parasite in IFAT assays. Only two out of six immunized animals became infected after P. vivax sporozoite challenge as compared with four out of six animals from the control group. These results suggest that this TRAP fragment has protective potential against P. vivax malaria and deserves further studies as vaccine candidate.
Mots-clé
Animals Antibodies, Protozoan/blood/immunology Aotidae Enzyme-Linked Immunosorbent Assay Female Fluorescent Antibody Technique, Indirect Malaria Vaccines/administration & dosage/*immunology Malaria, Vivax/*immunology/prevention & control Male Mice Mice, Inbred BALB C Peptide Fragments/immunology Pilot Projects Plasmodium vivax/*immunology Protozoan Proteins/*immunology Vaccines, Synthetic/administration & dosage/*immunology
Pubmed
Web of science
Création de la notice
24/01/2008 14:55
Dernière modification de la notice
20/08/2019 15:58
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