Use of an automated multiple-locus, variable-number tandem repeat-based method for rapid and high-throughput genotyping of Staphylococcus aureus isolates

Détails

ID Serval
serval:BIB_D88284FC8C75
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Use of an automated multiple-locus, variable-number tandem repeat-based method for rapid and high-throughput genotyping of Staphylococcus aureus isolates
Périodique
Journal of Clinical Microbiology
Auteur(s)
Francois  P., Huyghe  A., Charbonnier  Y., Bento  M., Herzig  S., Topolski  I., Fleury  B., Lew  D., Vaudaux  P., Harbarth  S., van Leeuwen  W., van Belkum  A., Blanc  D. S., Pittet  D., Schrenzel  J.
ISSN
0095-1137
Statut éditorial
Publié
Date de publication
07/2005
Peer-reviewed
Oui
Volume
43
Numéro
7
Pages
3346-55
Notes
Evaluation Studies
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jul
Résumé
Fast and reliable genotyping methods that allow real-time epidemiological surveillance would be instrumental to monitoring of the spread of methicillin-resistant Staphylococcus aureus. We describe an automated variable-number tandem repeat-based method for the rapid genotyping of Staphylococcus aureus. Multiplex PCR amplifications with eight primer pairs that target gene regions with variable numbers of tandem repeats were resolved by microcapillary electrophoresis and automatically assessed by cluster analysis. This genotyping technique was evaluated for its discriminatory power and reproducibility with clinical isolates of various origins, including a panel of control strains previously characterized by several typing methods and collections from either long-term carriers or defined nosocomial outbreaks. All steps of this new procedure were developed to ensure a rapid turnaround time and moderate cost. The results obtained suggest that this rapid approach is a valuable tool for the genotyping of S. aureus isolates in real time.
Mots-clé
Automation Bacterial Proteins/*genetics *Bacterial Typing Techniques Disease Outbreaks Electrophoresis, Gel, Pulsed-Field Genotype Humans Methicillin Resistance Minisatellite Repeats/*genetics Polymerase Chain Reaction/*methods Reproducibility of Results Staphylococcal Infections/epidemiology/microbiology Staphylococcus aureus/*classification/drug effects/genetics Time Factors
Pubmed
Web of science
Open Access
Oui
Création de la notice
29/01/2008 15:20
Dernière modification de la notice
20/08/2019 15:58
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