Intraneuronal angiotensinergic system in rat and human dorsal root ganglia.

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Etat: Public
Version: de l'auteur
ID Serval
serval:BIB_D864CE5FC55B
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Intraneuronal angiotensinergic system in rat and human dorsal root ganglia.
Périodique
Regulatory Peptides
Auteur(s)
Patil Jaspal, Schwab Alexander, Nussberger Juerg, Schaffner Thomas, Saavedra Juan M., Imboden Hans
ISSN
1873-1686[electronic], 0167-0115[linking]
Statut éditorial
Publié
Date de publication
2010
Peer-reviewed
Oui
Volume
162
Numéro
1-3
Pages
90-98
Langue
anglais
Résumé
To elucidate the local formation of angiotensin II (Ang II) in the neurons of sensory dorsal root ganglia (DRG), we studied the expression of angiotensinogen (Ang-N)-, renin-, angiotensin converting enzyme (ACE)- and cathepsin D-mRNA, and the presence of protein renin, Ang II, Substance P and calcitonin gene-related peptide (CGRP) in the rat and human thoracic DRG. Quantitative real time PCR (qRT-PCR) studies revealed that rat DRG expressed substantial amounts of Ang-N- and ACE mRNA, while renin mRNA as well as the protein renin were untraceable. Cathepsin D-mRNA and cathepsin D-protein were detected in the rat DRG indicating the possibility of existence of pathways alternative to renin for Ang I formation. Angiotensin peptides were successfully detected with high performance liquid chromatography and radioimmunoassay in human DRG extracts. In situ hybridization in rat DRG confirmed additionally expression of Ang-N mRNA in the cytoplasm of numerous neurons. Intracellular Ang II staining could be shown in number of neurons and their processes in both the rat and human DRG. Interestingly we observed neuronal processes with angiotensinergic synapses en passant, colocalized with synaptophysin, within the DRG. In the DRG, we also identified by qRT-PCR, expression of Ang II receptor AT(1A) and AT(2)-mRNA while AT(1B)-mRNA was not traceable. In some neurons Substance P and CGRP were found colocalized with Ang II. The intracellular localization and colocalization of Ang II with Substance P and CGRP in the DRG neurons may indicate a participation and function of Ang II in the regulation of nociception. In conclusion, these results suggest that Ang II may be produced locally in the neurons of rat and human DRG and act as a neurotransmitter.
Mots-clé
Renin-Angiotensin System, Angiotensin II, Neurotransmitter, Neuronal Angiotensin, Sensory System, Spontaneously Hypertensive-Rats, Receptor Messenger-RNA, II AT(1) Receptors, Substance-P, Spinal-Cord, Prophylactic Treatment, Trigeminal Ganglion, Sensory Neurons, Brain, Localization
Pubmed
Web of science
Création de la notice
29/06/2010 10:27
Dernière modification de la notice
20/08/2019 15:57
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