Storage and uptake of D-serine into astrocytic synaptic-like vesicles specify gliotransmission.

Détails

Ressource 1Télécharger: BIB_D85163C5EE09.P001.pdf (5107.46 [Ko])
Etat: Public
Version: de l'auteur
Document(s) secondaire(s)
Télécharger: 3413.full.pdf (3010.69 [Ko])
Etat: Public
Version: Final published version
ID Serval
serval:BIB_D85163C5EE09
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Storage and uptake of D-serine into astrocytic synaptic-like vesicles specify gliotransmission.
Périodique
Journal of Neuroscience
Auteur(s)
Martineau M., Shi T., Puyal J., Knolhoff A.M., Dulong J., Gasnier B., Klingauf J., Sweedler J.V., Jahn R., Mothet J.P.
ISSN
1529-2401 (Electronic)
ISSN-L
0270-6474
Statut éditorial
Publié
Date de publication
2013
Peer-reviewed
Oui
Volume
33
Numéro
8
Pages
3413-3423
Langue
anglais
Résumé
Glial cells are increasingly recognized as active players that profoundly influence neuronal synaptic transmission by specialized signaling pathways. In particular, astrocytes have been shown recently to release small molecules, such as the amino acids l-glutamate and d-serine as "gliotransmitters," which directly control the efficacy of adjacent synapses. However, it is still controversial whether gliotransmitters are released from a cytosolic pool or by Ca(2+)-dependent exocytosis from secretory vesicles, i.e., by a mechanism similar to the release of synaptic vesicles in synapses. Here we report that rat cortical astrocytes contain storage vesicles that display morphological and biochemical features similar to neuronal synaptic vesicles. These vesicles share some, but not all, membrane proteins with synaptic vesicles, including the SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) synaptobrevin 2, and contain both l-glutamate and d-serine. Furthermore, they show uptake of l-glutamate and d-serine that is driven by a proton electrochemical gradient. d-Serine uptake is associated with vesicle acidification and is dependent on chloride. Whereas l-serine is not transported, serine racemase, the synthesizing enzyme for d-serine, is anchored to the membrane of the vesicles, allowing local generation of d-serine. Finally, we reveal a previously unexpected mutual vesicular synergy between d-serine and l-glutamate filling in glia vesicles. We conclude that astrocytes contain vesicles capable of storing and releasing d-serine, l-glutamate, and most likely other neuromodulators in an activity-dependent manner.
Mots-clé
Animals, Astrocytes/metabolism, Astrocytes/ultrastructure, Biological Transport, Active/physiology, Cells, Cultured, Female, Male, Neuroglia/metabolism, Neuroglia/physiology, Rats, Rats, Sprague-Dawley, Rats, Wistar, Serine/metabolism, Synaptic Transmission/physiology, Synaptic Vesicles/metabolism, Synaptic Vesicles/ultrastructure
Pubmed
Web of science
Open Access
Oui
Création de la notice
01/07/2013 10:07
Dernière modification de la notice
20/08/2019 15:57
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