Array CGH analysis in primary gastrointestinal stromal tumors: cytogenetic profile correlates with anatomic site and tumor aggressiveness, irrespective of mutational status

Détails

ID Serval
serval:BIB_D84F85A7FF5F
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Array CGH analysis in primary gastrointestinal stromal tumors: cytogenetic profile correlates with anatomic site and tumor aggressiveness, irrespective of mutational status
Périodique
Genes Chromosomes and Cancer
Auteur(s)
Wozniak  A., Sciot  R., Guillou  L., Pauwels  P., Wasag  B., Stul  M., Vermeesch  J. R., Vandenberghe  P., Limon  J., biec-Rychter  M.
ISSN
1045-2257 (Print)
Statut éditorial
Publié
Date de publication
2007
Volume
46
Numéro
3
Pages
261-276
Notes
PT - Journal Article PT - Research Support, Non-U.S. Gov't
Résumé
Gastrointestinal stromal tumors (GISTs) comprise a biologically diverse group of neoplasms with respect to activating mutations in either KIT or PDGFRA, histology, anatomical site of origin, and clinical aggressiveness. In this study, we applied the high resolution array-based comparative genomic hybridization (array-CGH) technology to 66 primary GISTs (40 gastric and 26 nongastric, 48 with KIT and 18 with PDGFRA mutations) for identification of novel high-level alterations and for characterization of genotype-related genomic changes. All cases had genomic imbalances with the highest occurrence of 14q (73%), 1p (62%), 22q (59%), 15q (38%), and 13q (29%) losses. Our data indicate that loss of chromosome 14 and/or 22 is an early change in GIST tumorigenesis irrespective of tumor genotype. Furthermore, DNA copy number changes showed a site dependent pattern. These included lower incidence of losses at 14q (87% vs. 35%), and higher frequency of losses at 1p (45% vs. 85%) and 15q (17% vs. 69%) in nongastric versus gastric site (P<0.001 for all). However, in the multivariate analysis with adjustment to tumor risk stratification, only the 14q loss site-dependent pattern of distribution retained its significance. These findings suggest that loss of 14q is a relatively less frequent genetic event in the development of nongastric GISTs, the lack of which is most likely substituted by the accumulation of 1p/15q and other changes. The novel minimal overlapping regions of deletion at 1p (1p36.32-1p35.2, 1p34.1, and 1p22.1-1p21.3), 13q (13q14.11-q14.2 and 13q32.3-q33.1), and 15q23 were delineated, which point to chromosomal regions that may harbor genes relevant to the development of these neoplasms
Mots-clé
Adult/Aged/Aged,80 and over/Chromosome Aberrations/Cytogenetic Analysis/Female/Gastrointestinal Stromal Tumors/genetics/metabolism/Pathology/Gene Dosage/Humans/Male/Middle Aged/Mutation/Oligonucleotide Array Sequence Analysis/Proto-Oncogene Proteins c-kit/Receptor,Platelet-Derived Growth Factor alpha
Pubmed
Web of science
Création de la notice
29/01/2008 18:34
Dernière modification de la notice
20/08/2019 15:57
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