Single tube competitive PCR for quantitation of CMV DNA in the blood of HIV+ and solid organ transplant patients

Détails

ID Serval
serval:BIB_D849384907BE
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Single tube competitive PCR for quantitation of CMV DNA in the blood of HIV+ and solid organ transplant patients
Périodique
Journal of Virological Methods
Auteur(s)
Chatellard  P., Sahli  R., Iten  A., von Overbeck  J., Meylan  P. R.
ISSN
0166-0934 (Print)
Statut éditorial
Publié
Date de publication
04/1998
Volume
71
Numéro
2
Pages
137-46
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Apr
Résumé
Cytomegalovirus (CMV) infection is a major cause of morbidity and mortality in transplant and HIV-infected patients. However, CMV can also cause asymptomatic infection. An elevated blood viral load as assessed by various methods appears to be a predictor for symptomatic infections, and can be used to identify patients at the highest risk of developing CMV disease. We developed a single tube competitive quantitative PCR assay for CMV DNA, using as a competitor a plasmid carrying the target sequence for amplification with an internal deletion. The analysis of data from repeated extractions and amplifications of samples showed that the coefficient of variation of the assay was typically less than 20%. Clinical samples from 14 HIV-infected and 13 solid organ transplant patients were analyzed. Widely varying CMV DNA levels were found in leukocytes, with a positive correlation with the measure of infectivity in the leukocytes by quantitative culture on fibroblasts. The highest CMV DNA content in leukocytes was found in two patients with presumptive CMV disease. In HIV patients, the amount of DNA in leukocytes was much larger than in solid organ transplant recipients, when standardized for infectivity. Although based on a very limited number of patients, this observation probably points to a difference in the biology of CMV infection in these two categories of susceptible individuals. CMV DNA was also found in the plasma of some of the patients with a high CMV DNA leukocyte load. The present test should be useful for identifying patients at high risk of developing CMV disease, for monitoring therapeutic efficacy of antiviral regimens and to improve the understanding the pathogenesis of CMV infection.
Mots-clé
AIDS-Related Opportunistic Infections/*diagnosis Cytomegalovirus/genetics/*isolation & purification/physiology Cytomegalovirus Infections/*diagnosis DNA, Viral/*blood Humans Leukocytes/virology *Organ Transplantation Polymerase Chain Reaction/*methods Postoperative Complications/diagnosis Predictive Value of Tests Viral Load Viremia/diagnosis
Pubmed
Web of science
Création de la notice
24/01/2008 21:51
Dernière modification de la notice
20/08/2019 16:57
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