Combining Standard Molecular Typing and Whole Genome Sequencing to Investigate Pseudomonas aeruginosa Epidemiology in Intensive Care Units.

Détails

Ressource 1Télécharger: Magalhães_2020_FrontPublicHealth_Combining Standard Molecular Typing and WGSg to Investigate P aeruginosa Epidemiology in ICU.pdf (2042.70 [Ko])
Etat: Public
Version: de l'auteur
Licence: CC BY 4.0
ID Serval
serval:BIB_D83F2D6A3BC0
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Combining Standard Molecular Typing and Whole Genome Sequencing to Investigate Pseudomonas aeruginosa Epidemiology in Intensive Care Units.
Périodique
Frontiers in public health
Auteur(s)
Magalhães B., Valot B., Abdelbary MMH, Prod'hom G., Greub G., Senn L., Blanc D.S.
ISSN
2296-2565 (Print)
ISSN-L
2296-2565
Statut éditorial
Publié
Date de publication
2020
Peer-reviewed
Oui
Volume
8
Pages
3
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: epublish
Résumé
Pseudomonas aeruginosa is one of the main pathogens responsible for nosocomial infections, particularly in Intensive Care Units (ICUs). Due to the complexity of P. aeruginosa ecology, only powerful typing methods can efficiently allow its surveillance and the detection during expanding outbreaks. An increase in P. aeruginosa incidence was observed in the ICUs of the Lausanne University Hospital between 2010 and 2014. All clinical and environmental isolates retrieved during this period were typed with Double locus sequence typing (DLST), which detected the presence of three major genotypes: DLST 1-18, DLST 1-21, and DLST 6-7. DLST 1-18 (ST1076) isolates were previously associated with an epidemiologically well-described outbreak in the burn unit. Nevertheless, DLST 1-21 (ST253) and DLST 6-7 (ST17) showed sporadic occurrence with only few cases of possible transmission between patients. Whole genome sequencing (WGS) was used to further investigate the epidemiology of these three major P. aeruginosa genotypes in the ICUs. WGS was able to differentiate between outbreak and non-outbreak isolates and confirm suspected epidemiological links. Additionally, whole-genome single nucleotide polymorphisms (SNPs) results considered isolates as closely related for which no epidemiological links were suspected, expanding the epidemiological investigation to unsuspected links. The combination of a first-line molecular typing tool (DLST) with a more discriminatory method (WGS) proved to be an accurate and cost-efficient typing strategy for the investigation of P. aeruginosa epidemiology in the ICUs.
Mots-clé
Pseudomonas aeruginosa, double locus sequence typing, genomic epidemiology, molecular epidemiology, molecular typing, whole genome sequencing
Pubmed
Open Access
Oui
Création de la notice
14/02/2020 17:31
Dernière modification de la notice
21/02/2020 8:10
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