Gene expression profiling of rat spermatogonia and Sertoli cells reveals signaling pathways from stem cells to niche and testicular cancer cells to surrounding stroma.

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Ressource 1Télécharger: BIB_D7D4B13509A5.P001.pdf (1396.43 [Ko])
Etat: Public
Version: de l'auteur
ID Serval
serval:BIB_D7D4B13509A5
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Gene expression profiling of rat spermatogonia and Sertoli cells reveals signaling pathways from stem cells to niche and testicular cancer cells to surrounding stroma.
Périodique
Bmc Genomics
Auteur(s)
Ryser S., Glauser D., Vigier M., Zhang Y.Q., Tachini P., Schlegel W., Durand P., Irminger-Finger I.
ISSN
1471-2164[electronic], 1471-2164[linking]
Statut éditorial
Publié
Date de publication
2011
Volume
12
Pages
29
Langue
anglais
Résumé
Background: Stem cells and their niches are studied in many systems, but mammalian germ stem cells (GSC) and their niches are still poorly understood. In rat testis, spermatogonia and undifferentiated Sertoli cells proliferate before puberty, but at puberty most spermatogonia enter spermatogenesis, and Sertoli cells differentiate to support this program. Thus, pre-pubertal spermatogonia might possess GSC potential and pre-pubertal Sertoli cells niche functions. We hypothesized that the different stem cell pools at pre-puberty and maturity provide a model for the identification of stem cell and niche-specific genes. We compared the transcript profiles of spermatogonia and Sertoli cells from pre-pubertal and pubertal rats and examined how these related to genes expressed in testicular cancers, which might originate from inappropriate communication between GSCs and Sertoli cells.
Results: The pre-pubertal spermatogonia-specific gene set comprised known stem cell and spermatogonial stem cell (SSC) markers. Similarly, the pre-pubertal Sertoli cell-specific gene set comprised known niche gene transcripts. A large fraction of these specifically enriched transcripts encoded trans-membrane, extra-cellular, and secreted proteins highlighting stem cell to niche communication. Comparing selective gene sets established in this study with published gene expression data of testicular cancers and their stroma, we identified sets expressed genes shared between testicular tumors and pre-pubertal spermatogonia, and tumor stroma and pre-pubertal Sertoli cells with statistic significance.
Conclusions: Our data suggest that SSC and their niche specifically express complementary factors for cell communication and that the same factors might be implicated in the communication between tumor cells and their micro-enviroment in testicular cancer.
Pubmed
Web of science
Open Access
Oui
Création de la notice
15/03/2011 15:35
Dernière modification de la notice
20/08/2019 16:57
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