The primary in vivo immune response to Mls-1 (Mtv-7 sag). Route of injection determines the immune response pattern.

Détails

ID Serval
serval:BIB_D7CDD3D29A7D
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
The primary in vivo immune response to Mls-1 (Mtv-7 sag). Route of injection determines the immune response pattern.
Périodique
Immunology
Auteur⸱e⸱s
Andersson M., Acha-Orbea H.
ISSN
0019-2805 (Print)
ISSN-L
0019-2805
Statut éditorial
Publié
Date de publication
1994
Volume
83
Numéro
3
Pages
438-443
Langue
anglais
Résumé
Injection of cells expressing the retroviral superantigen Mls-1 (Mtv-7 sag) into adult Mls-1- mice induces a strong immune response including both T- and B-cell activation. This model was used for studying qualitative aspects of the immune response in normal mice with a defined antigen-presenting cell (the B cell) and without the use of adjuvant. BALB/c mice were injected locally or systemically with Mls-1-expressing spleen cells from Mls-1-congenic BALB.D2 mice. Intravenous injection led to an initially strong expansion of Mls-1-reactive V beta 6+ CD4+ cells mainly in the spleen, to a large degree explained by the trapping of reactive cells, and a rapid down-regulation of interleukin-2 (IL-2) and interferon-gamma (IFN-gamma) production, consistent with the proposed tolerogenic property of B cells as antigen-presenting cells. However, these mice developed a slowly appearing but persistent B-cell response dominated by IgG1-producing cells, suggesting a shift in lymphokines produced rather than complete unresponsiveness. Subcutaneous injection into the hind footpad with the same number of cells led to a strong local response in the draining lymph node, characterized by a dramatic increase of V beta 6+ CD4+ T cells, local production of IL-2 and IFN-gamma and a strong but short-lived antibody response dominated by IgG2a-producing cells, characteristic of a T-helper type 1 (Th1) type of response. Both routes of injection led ultimately to deletion of reactive T cells and anergy, as defined by the inability to produce IL-2 upon in vitro stimulation with Mls-1. It is concluded that Mls-1 presented by B cells induces qualitatively different responses in vivo dependent on the route of injection. We propose that the different responses result from the migration of the injected cells to different micro-anatomical sites in the lymphoid tissue. Furthermore, these results suggest that B cells may function as professional antigen-presenting cells in vivo present in an appropriate environment.
Mots-clé
Animals, Antibody Formation, CD4-Positive T-Lymphocytes/immunology, Cell Transplantation, Immunization, Immunoglobulins/metabolism, Injections, Intravenous, Injections, Subcutaneous, Interferon-gamma/secretion, Interleukin-2/secretion, Mice, Mice, Inbred BALB C, Retroviridae/immunology, Spleen/cytology, Spleen/immunology, Superantigens/administration &amp, dosage
Pubmed
Web of science
Création de la notice
24/01/2008 14:47
Dernière modification de la notice
20/08/2019 15:57
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