CozEa and CozEb play overlapping and essential roles in controlling cell division in Staphylococcus aureus.

Détails

ID Serval
serval:BIB_D6CE85156B3D
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
CozEa and CozEb play overlapping and essential roles in controlling cell division in Staphylococcus aureus.
Périodique
Molecular microbiology
Auteur(s)
Stamsås G.A., Myrbråten I.S., Straume D., Salehian Z., Veening J.W., Håvarstein L.S., Kjos M.
ISSN
1365-2958 (Electronic)
ISSN-L
0950-382X
Statut éditorial
Publié
Date de publication
09/2018
Peer-reviewed
Oui
Volume
109
Numéro
5
Pages
615-632
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Staphylococcus aureus needs to control the position and timing of cell division and cell wall synthesis to maintain its spherical shape. We identified two membrane proteins, named CozEa and CozEb, which together are important for proper cell division in S. aureus. CozEa and CozEb are homologs of the cell elongation regulator CozE <sup>Spn</sup> of Streptococcus pneumoniae. While cozEa and cozEb were not essential individually, the ΔcozEaΔcozEb double mutant was lethal. To study the functions of cozEa and cozEb, we constructed a CRISPR interference (CRISPRi) system for S. aureus, allowing transcriptional knockdown of essential genes. CRISPRi knockdown of cozEa in the ΔcozEb strain (and vice versa) causes cell morphological defects and aberrant nucleoid staining, showing that cozEa and cozEb have overlapping functions and are important for normal cell division. We found that CozEa and CozEb interact with and possibly influence localization of the cell division protein EzrA. Furthermore, the CozE-EzrA interaction is conserved in S. pneumoniae, and cell division is mislocalized in cozE <sup>Spn</sup> -depleted S. pneumoniae cells. Together, our results show that CozE proteins mediate control of cell division in S. aureus and S. pneumoniae, likely via interactions with key cell division proteins such as EzrA.
Mots-clé
Bacterial Proteins/genetics, Bacterial Proteins/physiology, Cell Cycle Proteins/genetics, Cell Cycle Proteins/metabolism, Cell Division/genetics, Clustered Regularly Interspaced Short Palindromic Repeats/genetics, DNA, Bacterial/genetics, Gene Knockdown Techniques, Membrane Proteins/genetics, Membrane Proteins/physiology, Mutation, Staphylococcus aureus/genetics, Staphylococcus aureus/pathogenicity, Staphylococcus aureus/physiology
Pubmed
Web of science
Création de la notice
25/06/2018 11:42
Dernière modification de la notice
20/08/2019 16:56
Données d'usage