Analytical interference of 4-hydroxy-3-methoxymethamphetamine with the measurement of plasma free normetanephrine by ultra-high pressure liquid chromatography-tandem mass spectrometry.

Détails

ID Serval
serval:BIB_D6BBBADD3A07
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Analytical interference of 4-hydroxy-3-methoxymethamphetamine with the measurement of plasma free normetanephrine by ultra-high pressure liquid chromatography-tandem mass spectrometry.
Périodique
Clinical Biochemistry
Auteur(s)
Dunand M., Donzelli M., Rickli A., Hysek C.M., Liechti M.E., Grouzmann E.
ISSN
1873-2933 (Electronic)
ISSN-L
0009-9120
Statut éditorial
Publié
Date de publication
2014
Peer-reviewed
Oui
Volume
47
Numéro
12
Pages
1121-1123
Langue
anglais
Notes
Publication types: Journal ArticlePublication Status: ppublish
Résumé
OBJECTIVES: The diagnosis of pheochromocytoma relies on the measurement of plasma free metanephrines assay whose reliability has been considerably improved by ultra-high pressure liquid chromatography tandem mass spectrometry (UHPLC-MS/MS). Here we report an analytical interference occurring between 4-hydroxy-3-methoxymethamphetamine (HMMA), a metabolite of 3,4-methylenedioxymethamphetamine (MDMA, "Ecstasy"), and normetanephrine (NMN) since they share a common pharmacophore resulting in the same product ion after fragmentation.
DESIGN AND METHODS: Synthetic HMMA was spiked into plasma samples containing various concentrations of NMN and the intensity of the interference was determined by UPLC-MS/MS before and after improvement of the analytical method.
RESULTS: Using a careful adjustment of chromatographic conditions including the change of the UPLC analytical column, we were able to distinguish both compounds. HMMA interference for NMN determination should be seriously considered since MDMA activates the sympathetic nervous system and if confounded with NMN may lead to false-positive tests when performing a differential diagnostic of pheochromocytoma.
Pubmed
Web of science
Création de la notice
25/09/2014 16:58
Dernière modification de la notice
20/08/2019 15:56
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