Induction of multiple CD8+ T cell responses against the inducible Hsp70 employing an Hsp70 oligoepitope peptide.

Détails

ID Serval
serval:BIB_D659D6572278
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Induction of multiple CD8+ T cell responses against the inducible Hsp70 employing an Hsp70 oligoepitope peptide.
Périodique
Oncology Reports
Auteur⸱e⸱s
Faure O., Graff-Dubois S., Alves P.M., Cornet S., Duffour M.T., Scardino A., Gross D.A., Miconnet I., Salcedo M., Chouaib S., Lemonnier F.A., Abastado J.P., Kosmatopoulos K.
ISSN
1021-335X
Statut éditorial
Publié
Date de publication
03/2007
Peer-reviewed
Oui
Volume
17
Numéro
3
Pages
679-685
Langue
anglais
Résumé
The inducible heat shock protein Hsp70 has been described as a tumour antigen being frequently overexpressed in tumours of various histologic origins, with a role in tumourigenicity, as a critical event in tumour progression. A strategy to enhance the immune response to an antigen is the identification of multiple epitopes and the induction of a polyspecific response. Applied to tumour vaccination, such a polyspecific response should lead to a more robust antitumour efficacy. The long peptide Hsp70380-402 encompasses three nonamer peptides with a high affinity for HLA-A *0201. In a previous paper, we have shown that two of these nonamer peptides, p391 and p393, can raise CTL to recognize tumour cells overexpressing Hsp70. In the present paper, we demonstrate that the third nonamer peptide, p380, is a new epitope efficient in raising an antitumour immune response. The p380-402 polypeptide was able to induce an immune response against each of the three constituent epitopes both in vivo in HLA-A *0201 transgenic mice and in vitro with human PBMC. This polypeptide therefore constitutes an interesting candidate for the induction of multiple HLA-A *0201-restricted anti-Hsp70 antitumour CTL responses.
Mots-clé
Animals, Antigens, Neoplasm, Blotting, Western, CD8-Positive T-Lymphocytes, COS Cells, Cercopithecus aethiops, Epitopes, T-Lymphocyte, HLA-A Antigens, HSP70 Heat-Shock Proteins, Humans, Mice, Mice, Transgenic, Peptides, Transfection
Pubmed
Web of science
Création de la notice
25/01/2008 15:08
Dernière modification de la notice
20/08/2019 15:56
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