Checkpoint kinases and the INO80 nucleosome remodeling complex enhance global chromatin mobility in response to DNA damage.

Détails

ID Serval
serval:BIB_D606E3424DF5
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Checkpoint kinases and the INO80 nucleosome remodeling complex enhance global chromatin mobility in response to DNA damage.
Périodique
Genes and Development
Auteur⸱e⸱s
Seeber A., Dion V., Gasser S.M.
ISSN
1549-5477 (Electronic)
ISSN-L
0890-9369
Statut éditorial
Publié
Date de publication
2013
Volume
27
Numéro
18
Pages
1999-2008
Langue
anglais
Résumé
Double-strand break repair by recombination requires a homology search. In yeast, induced breaks move significantly more than undamaged loci. To examine whether DNA damage provokes an increase in chromatin mobility generally, we tracked undamaged loci under DNA-damaging conditions. We found that the yeast checkpoint factors Mec1, Rad9, and Rad53 are required for genome-wide increases in chromatin mobility, but not the repair protein Rad51. Mec1 activation by targeted Ddc1/Ddc2 enhances chromatin mobility even in the absence of damage. Finally, the INO80 chromatin remodeler is shown to act downstream from Mec1 to increase chromatin mobility, highlighting an additional damage-related role of this nucleosome remodeling complex.
Mots-clé
Cell Cycle Checkpoints/physiology, Cell Cycle Proteins/genetics, Cell Cycle Proteins/metabolism, Chromatin/metabolism, DNA Damage, Genome, Fungal/genetics, Models, Biological, Phosphotransferases/metabolism, Saccharomyces cerevisiae/enzymology, Saccharomyces cerevisiae/genetics, Saccharomyces cerevisiae Proteins/genetics, Saccharomyces cerevisiae Proteins/metabolism
Pubmed
Web of science
Open Access
Oui
Création de la notice
12/02/2014 15:23
Dernière modification de la notice
20/08/2019 15:55
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