Crystal structure of the endosomal SNARE complex reveals common structural principles of all SNAREs.

Détails

ID Serval
serval:BIB_D5A3523DCEFB
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Crystal structure of the endosomal SNARE complex reveals common structural principles of all SNAREs.
Périodique
Nature Structural Biology
Auteur(s)
Antonin W., Fasshauer D., Becker S., Jahn R., Schneider T.R.
ISSN
1072-8368 (Print)
ISSN-L
1072-8368
Statut éditorial
Publié
Date de publication
2002
Peer-reviewed
Oui
Volume
9
Numéro
2
Pages
107-111
Langue
anglais
Résumé
SNARE proteins are crucial for intracellular membrane fusion in all eukaryotes. These proteins assemble into tight complexes that connect membranes and may induce fusion. The crystal structure of the neuronal core complex is represented by an unusually long bundle of four alpha-helices connected by 16 layers of mostly hydrophobic amino acids. Here we report the 1.9 A resolution crystal structure of an endosomal SNARE core complex containing four SNAREs: syntaxin 7, syntaxin 8, vti1b and endobrevin/VAMP-8. Despite limited sequence homology, the helix alignment and the layer structure of the endosomal complex are remarkably similar to those of the neuronal complex. However, subtle variations are evident that characterize different SNARE subfamilies. We conclude that the structure of the SNARE core complex is an evolutionarily conserved hallmark of all SNARE complexes and is intimately associated with the general role of SNAREs in membrane fusion.
Mots-clé
Amino Acid Sequence, Animals, Binding Sites, Crystallization, Crystallography, X-Ray, Endosomes/chemistry, Evolution, Molecular, Macromolecular Substances, Membrane Proteins/chemistry, Membrane Proteins/metabolism, Mice, Models, Molecular, Molecular Sequence Data, Protein Binding, Protein Structure, Secondary, Protein Structure, Tertiary, Qa-SNARE Proteins, R-SNARE Proteins, Rats, SNARE Proteins, Sequence Alignment, Vesicular Transport Proteins
Pubmed
Web of science
Création de la notice
15/09/2011 9:26
Dernière modification de la notice
20/08/2019 15:55
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