Cytokine production after intravenous or peritoneal gram-negative bacterial challenge in mice. Comparative protective efficacy of antibodies to tumor necrosis factor-alpha and to lipopolysaccharide

Détails

ID Serval
serval:BIB_D5361A71CA4F
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Cytokine production after intravenous or peritoneal gram-negative bacterial challenge in mice. Comparative protective efficacy of antibodies to tumor necrosis factor-alpha and to lipopolysaccharide
Périodique
Journal of Immunology
Auteur(s)
Zanetti  G., Heumann  D., Gerain  J., Kohler  J., Abbet  P., Barras  C., Lucas  R., Glauser  M. P., Baumgartner  J. D.
ISSN
0022-1767
Statut éditorial
Publié
Date de publication
03/1992
Peer-reviewed
Oui
Volume
148
Numéro
6
Pages
1890-7
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Mar 15
Résumé
The production of TNF-alpha, IL-1, and IL-6 was measured in mice after bolus i.v. Escherichia coli O111 LPS injections and during bacteremia induced either by bolus i.v. or by i.p. challenges of live E. coli O111. High but transient TNF-alpha peaks were observed after bolus i.v. LPS or bacterial challenges. In contrast, the levels during lethal peritonitis increased progressively to values 50- to 100-fold lower than the peak values observed after i.v. injections, and remained sustained until death. Whereas after i.v. challenge with 1000 LD50 of LPS, anti-TNF-alpha antibody fully protected mice from death and reduced serum IL-1 and IL-6 levels, anti-TNF-alpha antibody did not improve the survival of mice nor reduced serum IL-1 and IL-6 levels after i.p. bacterial challenge. In contrast to anti-TNF-alpha antibodies, anti-LPS antibodies were protective in the peritonitis model. Protection was accompanied by a striking reduction of bacterial numbers and of TNF-alpha, IL-1, and IL-6 levels in the serum, but the levels of these cytokines were only marginally affected in the peritoneal lavage fluid. This latter observation demonstrates that the local peritoneal cytokines did not diffuse readily into the circulation, thus suggesting that at least part of the circulating cytokines are produced systemically. In conclusion, the striking differences between cytokine profiles as well as the divergent efficacy of anti-TNF-alpha antibody after i.v. bolus and after i.p. challenges suggest that TNF-alpha may not be as important in the pathogenesis of lethal peritonitis than after lethal acute bacteremia.
Mots-clé
Animals Cytokines/*biosynthesis Escherichia coli Infections/*physiopathology Female Interleukin-1/immunology Interleukin-6/immunology Lipopolysaccharides/*immunology Mice Peritonitis/*immunology Time Factors Tumor Necrosis Factor-alpha/*immunology
Pubmed
Web of science
Création de la notice
21/01/2008 11:04
Dernière modification de la notice
20/08/2019 16:55
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