Control of transcription elongation by GreA determines rate of gene expression in Streptococcus pneumoniae.

Détails

ID Serval
serval:BIB_D51FFCF6BED6
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Control of transcription elongation by GreA determines rate of gene expression in Streptococcus pneumoniae.
Périodique
Nucleic Acids Research
Auteur(s)
Yuzenkova Y., Gamba P., Herber M., Attaiech L., Shafeeq S., Kuipers O.P., Klumpp S., Zenkin N., Veening J.W.
ISSN
1362-4962 (Electronic)
ISSN-L
0305-1048
Statut éditorial
Publié
Date de publication
2014
Volume
42
Numéro
17
Pages
10987-10999
Langue
anglais
Résumé
Transcription by RNA polymerase may be interrupted by pauses caused by backtracking or misincorporation that can be resolved by the conserved bacterial Gre-factors. However, the consequences of such pausing in the living cell remain obscure. Here, we developed molecular biology and transcriptome sequencing tools in the human pathogen Streptococcus pneumoniae and provide evidence that transcription elongation is rate-limiting on highly expressed genes. Our results suggest that transcription elongation may be a highly regulated step of gene expression in S. pneumoniae. Regulation is accomplished via long-living elongation pauses and their resolution by elongation factor GreA. Interestingly, mathematical modeling indicates that long-living pauses cause queuing of RNA polymerases, which results in 'transcription traffic jams' on the gene and thus blocks its expression. Together, our results suggest that long-living pauses and RNA polymerase queues caused by them are a major problem on highly expressed genes and are detrimental for cell viability. The major and possibly sole function of GreA in S. pneumoniae is to prevent formation of backtracked elongation complexes.
Mots-clé
Bacterial Proteins/physiology, Gene Expression Regulation, Bacterial, Models, Genetic, Promoter Regions, Genetic, Streptococcus pneumoniae/cytology, Streptococcus pneumoniae/genetics, Transcription Elongation, Genetic, Transcription Initiation, Genetic, Transcriptional Elongation Factors/physiology
Pubmed
Web of science
Open Access
Oui
Création de la notice
11/10/2016 16:33
Dernière modification de la notice
20/08/2019 16:54
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