Absence of p53 gene mutations in a tumor panel representative of pilocytic astrocytoma diversity using a p53 functional assay.

Détails

ID Serval
serval:BIB_D50B7ABC0660
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Absence of p53 gene mutations in a tumor panel representative of pilocytic astrocytoma diversity using a p53 functional assay.
Périodique
International journal of cancer. Journal international du cancer
Auteur(s)
Ishii N., Sawamura Y., Tada M., Daub D.M., Janzer R.C., Meagher-Villemure M., de Tribolet N., Van Meir E.G.
ISSN
0020-7136
Statut éditorial
Publié
Date de publication
1998
Volume
76
Numéro
6
Pages
797-800
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't - Publication Status: ppublish
Résumé
Although p53-gene mutations occur with significant frequency in diffuse low-grade and high-grade astrocytomas, and are postulated to play an important role in tumorigenesis in these cases, the role of the p53 gene in pilocytic astrocytomas remains unclear. Published data using DNA-based assays for p53-gene analysis in these tumors have shown contradictory results in mutation frequency (0-14%). It is not known whether these heterogeneous results stem from the biological diversity of this tumor group or from technical problems. To re-evaluate p53-gene status in pilocytic tumors, we analyzed 18 tumors chosen to represent the clinical and biological heterogeneity of this tumor type with respect to anatomical location, patient age, gender, ethnic origin (Caucasian or Japanese) and the concomitant occurrence of neurofibromatosis type 1 (NF1). All primary tumors were histologically diagnosed as pilocytic astrocytoma (WHO grade I), except for one anaplastic pilocytic astrocytoma (WHO grade III) which developed in an NF1 patient and recurred as glioblastoma multiforme (WHO grade IV). p53 mutations were detected using an assay in yeast which tests the transcriptional activity of p53 proteins synthesized from tumor mRNA-derived p53-cDNA templates. None of 18 tumors, including 3 NF1-related tumors, showed p53-gene mutations between and including exons 4 and 11. We conclude that p53-gene mutations are extremely rare findings in pilocytic astrocytomas, and are absent even in those exceptional cases in which malignant progression of such tumors has occurred.
Mots-clé
Adolescent, Adult, Astrocytoma, Brain Neoplasms, Child, Child, Preschool, Female, Genes, p53, Humans, Infant, Male, Middle Aged, Mutation
Pubmed
Web of science
Création de la notice
29/01/2008 19:36
Dernière modification de la notice
20/08/2019 16:54
Données d'usage