Compartmental analysis of steady-state taurocholate transport kinetics by isolated rat hepatocytes

Détails

ID Serval
serval:BIB_D50656F3CFB3
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Compartmental analysis of steady-state taurocholate transport kinetics by isolated rat hepatocytes
Périodique
Hepatology
Auteur(s)
Coche  T., Deroubaix  X., Depiereux  E., Feytmans  E.
ISSN
0270-9139 (Print)
Statut éditorial
Publié
Date de publication
06/1991
Volume
13
Numéro
6
Pages
1203-14
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jun
Résumé
We used compartmental modeling to describe taurocholate transport by isolated rat liver cells in suspension. Cells are preincubated in the presence of unlabeled taurocholate. When a steady-state for taurocholate is reached, radiolabeled taurocholate is added to the medium and its exchange kinetics between the medium and the cells are followed over time. Because the studies are performed under steady-state conditions, the kinetics can be described by linear compartmental models. We found a closed two-compartment model sufficient to describe the steady-state transport data. Simulations reveal that if the pools of free and bound intracellular taurocholate exchange rapidly, the cells will behave as a single, kinetically homogeneous compartment and intracellular events will not influence the exchange kinetics of taurocholate between the medium and the cells. The two-compartment model was used to study the concentration dependence of taurocholate transport by isolated cells. Steady-state transport rates and taurocholate concentrations in the medium and the cells were calculated using the model equations. Taurocholate influx, accumulation and efflux processes were studied simultaneously by examining the relationship between appropriate combinations of these variables. Application of this approach to study the inhibition of taurocholate transport by taurochenodeoxycholate is illustrated. In conclusion, this method provides a complementary approach to initial rate studies, which are generally used to investigate bile acid transport by isolated cells.
Mots-clé
Animals Biological Transport Cell Separation Homeostasis Liver/cytology/*metabolism Methods Models, Biological Taurochenodeoxycholic Acid/pharmacology Taurocholic Acid/antagonists & inhibitors/*pharmacokinetics
Pubmed
Web of science
Création de la notice
28/01/2008 12:03
Dernière modification de la notice
20/08/2019 16:54
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