Assessment of immunogenicity of human Melan-A peptide analogues in HLA-A*0201/Kb transgenic mice

Détails

ID Serval
serval:BIB_D50578F8511D
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Assessment of immunogenicity of human Melan-A peptide analogues in HLA-A*0201/Kb transgenic mice
Périodique
Journal of Immunology
Auteur⸱e⸱s
Men  Y., Miconnet  I., Valmori  D., Rimoldi  D., Cerottini  J. C., Romero  P.
ISSN
0022-1767 (Print)
Statut éditorial
Publié
Date de publication
03/1999
Volume
162
Numéro
6
Pages
3566-73
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Mar 15
Résumé
Previous studies have shown that substitution of single amino acid residues in human Melan-A immunodominant peptides Melan-A27-35 and Melan-A26-35 greatly improved their binding and the stability of peptide/HLA-A*0201 complexes. In particular, one Melan-A peptide analogue was more efficient in the generation of Melan-A peptide-specific and melanoma-reactive CTL than its parental peptide in vitro from human PBL. In this study, we analyzed the in vivo immunogenicity of Melan-A natural peptides and their analogues in HLA-A*0201/Kb transgenic mice. We found that two human Melan-A natural peptides, Melan-A26-35 and Melan-A27-35, were relatively weak immunogens, whereas several Melan-A peptide analogues were potent immunogens for in vivo CTL priming. In addition, induced Melan-A peptide-specific mouse CTL cross-recognized natural Melan-A peptides and their analogues. More interestingly, these mouse CTL were also able to lyse human melanoma cell lines in vitro in a HLA-A*0201-restricted, Melan-A-specific manner. Our results indicate that the HLA-A*0201/Kb transgenic mouse is a useful animal model to perform preclinical testing of potential cancer vaccines, and that Melan-A peptide analogues are attractive candidates for melanoma immunotherapy.
Mots-clé
Alleles Animals Antigens, Neoplasm Arginine Cell Line Cytotoxicity, Immunologic/genetics Epitopes, T-Lymphocyte/immunology H-2 Antigens/*genetics HLA-A Antigens/*genetics Humans Injections, Subcutaneous Leucine Lymphocyte Activation/genetics Melanoma/immunology/metabolism Mice Mice, Transgenic Neoplasm Proteins/administration & dosage/*immunology/metabolism Oligopeptides/administration & dosage/*immunology/metabolism T-Lymphocytes, Cytotoxic/immunology/metabolism T-Lymphocytes, Helper-Inducer/immunology Tumor Cells, Cultured
Pubmed
Web of science
Création de la notice
28/01/2008 11:14
Dernière modification de la notice
20/08/2019 15:54
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