Innate immune responses of macrophages and dendritic cells to poxvirus : P199
Détails
ID Serval
serval:BIB_D4D3F0146A8C
Type
Actes de conférence (partie): contribution originale à la littérature scientifique, publiée à l'occasion de conférences scientifiques, dans un ouvrage de compte-rendu (proceedings), ou dans l'édition spéciale d'un journal reconnu (conference proceedings).
Sous-type
Poster: résume de manière illustrée et sur une page unique les résultats d'un projet de recherche. Les résumés de poster doivent être entrés sous "Abstract" et non "Poster".
Collection
Publications
Institution
Titre
Innate immune responses of macrophages and dendritic cells to poxvirus : P199
Titre de la conférence
Annual Joint Meeting of the Swiss Societies for Pneumology, Paediatric Pneumology, Allergology and Immunology, Thoracic Surgery
Adresse
Fribourg, April 17 and 18, 2008
ISBN
1424-7860
Statut éditorial
Publié
Date de publication
2008
Peer-reviewed
Oui
Volume
138
Série
Swiss Medical Weekly
Pages
46S
Langue
anglais
Notes
Background: Attenuated NYVAC and MVA poxvirus strains are currently used for vaccine development against a broad spectrum of diseases. Whilst these vectors have been shown to be immunogenic and safe in humans, the innate immune responses they elicit remain largely unknown.
Aim: To assess the response of human THP-1 cells and mouse dendritic cells to poxviruses infection and to elucidate the role of the Toll-like-receptor (TLR) pathways in that response.
Results: MVA-stimulated THP-1 monocytes secreted large amounts of chemokines (MIP-1, MCP-1, IP-10, RANTES), but low levels of proinflammatory cytokines (TNF, IL-6, IL-1beta). In contrast, NYVAC induced weak production of both cytokines and chemokines in THP-1 cells. Using wild-type and TLR2, TLR4, MyD88 and TRIF deficient mouse dendritic cells, we observed that TLR2-MyD88 was essential for production of chemokines, but dispensable for IFNbeta production, after stimulation with MVA.
Conclusion: MVA triggers innate immune responses of macrophages and dendritic cells via TLR-dependent and TLR-independent pathways. Work is in progress to identify the TLR-independent pathway(s) activated by MVA.
Aim: To assess the response of human THP-1 cells and mouse dendritic cells to poxviruses infection and to elucidate the role of the Toll-like-receptor (TLR) pathways in that response.
Results: MVA-stimulated THP-1 monocytes secreted large amounts of chemokines (MIP-1, MCP-1, IP-10, RANTES), but low levels of proinflammatory cytokines (TNF, IL-6, IL-1beta). In contrast, NYVAC induced weak production of both cytokines and chemokines in THP-1 cells. Using wild-type and TLR2, TLR4, MyD88 and TRIF deficient mouse dendritic cells, we observed that TLR2-MyD88 was essential for production of chemokines, but dispensable for IFNbeta production, after stimulation with MVA.
Conclusion: MVA triggers innate immune responses of macrophages and dendritic cells via TLR-dependent and TLR-independent pathways. Work is in progress to identify the TLR-independent pathway(s) activated by MVA.
Web of science
Création de la notice
13/10/2009 13:16
Dernière modification de la notice
20/08/2019 15:54
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