Mechanistic insight into a peptide hormone signaling complex mediating floral organ abscission.

Détails

ID Serval
serval:BIB_D493C09A9B1D
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Mechanistic insight into a peptide hormone signaling complex mediating floral organ abscission.
Périodique
eLife
Auteur⸱e⸱s
Santiago J., Brandt B., Wildhagen M., Hohmann U., Hothorn L.A., Butenko M.A., Hothorn M.
ISSN
2050-084X (Electronic)
ISSN-L
2050-084X
Statut éditorial
Publié
Date de publication
08/04/2016
Peer-reviewed
Oui
Volume
5
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Résumé
Plants constantly renew during their life cycle and thus require to shed senescent and damaged organs. Floral abscission is controlled by the leucine-rich repeat receptor kinase (LRR-RK) HAESA and the peptide hormone IDA. It is unknown how expression of IDA in the abscission zone leads to HAESA activation. Here we show that IDA is sensed directly by the HAESA ectodomain. Crystal structures of HAESA in complex with IDA reveal a hormone binding pocket that accommodates an active dodecamer peptide. A central hydroxyproline residue anchors IDA to the receptor. The HAESA co-receptor SERK1, a positive regulator of the floral abscission pathway, allows for high-affinity sensing of the peptide hormone by binding to an Arg-His-Asn motif in IDA. This sequence pattern is conserved among diverse plant peptides, suggesting that plant peptide hormone receptors may share a common ligand binding mode and activation mechanism.
Mots-clé
Arabidopsis/physiology, Arabidopsis Proteins/biosynthesis, Arabidopsis Proteins/chemistry, Arabidopsis Proteins/metabolism, Binding Sites, Crystallography, X-Ray, Flowers/physiology, Gene Expression Regulation, Plant, Protein Binding, Protein Conformation, Protein Kinases/metabolism, Protein-Serine-Threonine Kinases/biosynthesis, Protein-Serine-Threonine Kinases/chemistry, Signal Transduction, <i>a. thaliana</i>, biophysics, floral abscission, membrane signaling, peptide hormone, plant biology, plant development, protein complexes, receptor kinase, structural biology
Pubmed
Web of science
Open Access
Oui
Création de la notice
10/01/2019 17:18
Dernière modification de la notice
21/08/2019 5:35
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