A highly sensitive in vitro infection assay to explore early stages of mouse mammary tumor virus infection.
Détails
ID Serval
serval:BIB_D483B952A5E8
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
A highly sensitive in vitro infection assay to explore early stages of mouse mammary tumor virus infection.
Périodique
Journal of Virology
ISSN
0022-538X (Print)
ISSN-L
0022-538X
Statut éditorial
Publié
Date de publication
1997
Peer-reviewed
Oui
Volume
71
Numéro
10
Pages
7289-7294
Langue
anglais
Résumé
Mouse mammary tumor virus (MMTV) infection of adult mice induces a strong response to superantigen (Sag) in their draining lymph nodes, which results from the presentation of Sag by MMTV-infected B cells to Sag-reactive T cells. To date, infection with physiologically relevant doses of MMTV can be detected in vivo only after several days of Sag-mediated T-cell-dependent amplification of infected B cells. Furthermore, no efficient in vitro system of detecting MMTV infection is available. Such a model would allow the dissection of the early phase of infection, the assessment of the contributions of different cell types, and the screening of large panels of molecules for their potential roles in infection and Sag response. For these reasons, we have established an in vitro model for detecting infection which is as sensitive and reproducible as the in vivo model. We found that the viral envelope (Env) protein is crucial for target cell infection but not for presentation of Sag. Furthermore, we show that infection of purified B cells with MMTV induces entry of Sag-responsive T cells into the cell cycle, while other professional antigen-presenting cells, such as dendritic cells, are much less efficient in inducing a response.
Mots-clé
Animals, B-Lymphocytes/immunology, B-Lymphocytes/virology, Dendritic Cells/immunology, Female, Lymph Nodes/immunology, Lymphocyte Culture Test, Mixed, Mammary Neoplasms, Experimental/immunology, Mammary Neoplasms, Experimental/virology, Mammary Tumor Virus, Mouse/immunology, Mammary Tumor Virus, Mouse/isolation & purification, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Milk/virology, Retroviridae Infections/immunology, Sensitivity and Specificity, Spleen/immunology, Superantigens/immunology, T-Lymphocytes/immunology, T-Lymphocytes/virology, Tumor Virus Infections/immunology
Pubmed
Web of science
Création de la notice
24/01/2008 15:48
Dernière modification de la notice
20/08/2019 16:54