Fuzzy logic algorithm to extract specific interaction forces from atomic force microscopy data

Détails

ID Serval
serval:BIB_D470D465B36F
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Fuzzy logic algorithm to extract specific interaction forces from atomic force microscopy data
Périodique
Review of Scientific Instruments
Auteur(s)
Kasas S., Riederer B.M., Catsicas S., Cappella B., Dietler G.
ISSN
0034-6748
Statut éditorial
Publié
Date de publication
2000
Volume
71
Numéro
5
Pages
2082-2086
Langue
anglais
Résumé
The atomic force microscope is not only a very convenient tool for studying the topography of different samples, but it can also be used to measure specific binding forces between molecules. For this purpose, one type of molecule is attached to the tip and the other one to the substrate. Approaching the tip to the substrate allows the molecules to bind together. Retracting the tip breaks the newly formed bond. The rupture of a specific bond appears in the force-distance curves as a spike from which the binding force can be deduced. In this article we present an algorithm to automatically process force-distance curves in order to obtain bond strength histograms. The algorithm is based on a fuzzy logic approach that permits an evaluation of "quality" for every event and makes the detection procedure much faster compared to a manual selection. In this article, the software has been applied to measure the binding strength between tubuline and microtubuline associated proteins.
Web of science
Création de la notice
24/01/2008 15:34
Dernière modification de la notice
20/08/2019 16:54
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