Proton Motive Force Disruptors Block Bacterial Competence and Horizontal Gene Transfer.

Détails

ID Serval
serval:BIB_D3FFCCA23D11
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Proton Motive Force Disruptors Block Bacterial Competence and Horizontal Gene Transfer.
Périodique
Cell host & microbe
Auteur⸱e⸱s
Domenech A., Brochado A.R., Sender V., Hentrich K., Henriques-Normark B., Typas A., Veening J.W.
ISSN
1934-6069 (Electronic)
ISSN-L
1931-3128
Statut éditorial
Publié
Date de publication
08/04/2020
Peer-reviewed
Oui
Volume
27
Numéro
4
Pages
544-555.e3
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Streptococcus pneumoniae is a commensal of the human nasopharynx that can also cause severe antibiotic-resistant infections. Antibiotics drive the spread of resistance by inducing S. pneumoniae competence, in which bacteria express the transformation machinery that facilitates uptake of exogenous DNA and horizontal gene transfer (HGT). We performed a high-throughput screen and identified potent inhibitors of S. pneumoniae competence, called COM-blockers. COM-blockers limit competence by inhibiting the proton motive force (PMF), thereby disrupting export of a quorum-sensing peptide that regulates the transformation machinery. Known chemical PMF disruptors and alterations in pH homeostasis similarly inhibit competence. COM-blockers limit transformation of clinical multi-drug-resistant strains and HGT in infected mice. At their active concentrations, COM-blockers do not affect growth, compromise antibiotic activity, or elicit detectable resistance. COM-blockers provide an experimental tool to inhibit competence and other PMF-involved processes and could help reduce the spread of virulence factors and antibiotic resistance in bacteria. VIDEO ABSTRACT.
Mots-clé
DNA uptake, Streptococcus pneumoniae, antibiotic resistance, competence development, horizontal gene transfer, transformation
Pubmed
Web of science
Création de la notice
10/03/2020 16:17
Dernière modification de la notice
03/05/2020 6:02
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