A nuclear factor I-like activity and a liver-specific repressor govern estrogen-regulated in vitro transcription from the Xenopus laevis vitellogenin B1 promoter.

Détails

ID Serval
serval:BIB_D3D1E4C82CDB
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
A nuclear factor I-like activity and a liver-specific repressor govern estrogen-regulated in vitro transcription from the Xenopus laevis vitellogenin B1 promoter.
Périodique
Molecular and Cellular Biology
Auteur⸱e⸱s
Corthésy B., Cardinaux J.R., Claret F.X., Wahli W.
ISSN
0270-7306[print], 0270-7306[linking]
Statut éditorial
Publié
Date de publication
12/1989
Peer-reviewed
Oui
Volume
9
Numéro
12
Pages
5548-5562
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
A hormone-controlled in vitro transcription system derived from Xenopus liver nuclear extracts was exploited to identify novel cis-acting elements within the vitellogenin gene B1 promoter region. In addition to the already well-documented estrogen-responsive element (ERE), two elements were found within the 140 base pairs upstream of the transcription initiation site. One of them, a negative regulatory element, is responsible for the lack of promoter activity in the absence of the hormone and, as demonstrated by DNA-binding assays, interacts with a liver-specific transcription factor. The second is required in association with the estrogen-responsive element to mediate hormonal induction and is recognized by the Xenopus liver homolog of nuclear factor I.
Mots-clé
Animals, Base Sequence, CCAAT-Enhancer-Binding Proteins, Cell Line, DNA Probes, DNA-Binding Proteins/metabolism, Estrogens/physiology, Female, Gene Expression Regulation, Genes, Hela Cells/metabolism, Humans, Kidney/metabolism, Liver/metabolism, Male, Molecular Sequence Data, NFI Transcription Factors, Nuclear Proteins, Promoter Regions, Genetic, Repressor Proteins/metabolism, Sequence Homology, Nucleic Acid, Templates, Genetic, Transcription Factors/metabolism, Transcription, Genetic, Vitellogenins/genetics, Xenopus laevis, Y-Box-Binding Protein 1
Pubmed
Web of science
Création de la notice
18/02/2008 10:32
Dernière modification de la notice
20/08/2019 15:53
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